HPLC Analysis of Nateglinide on Purospher^® STAR RP-18 endcapped

column

Purospher STAR RP-18 endcapped 150 x 4.6 mm (1.51455.0001)

mobile phase

[A] 0.05% trifluoroacetic acid solution; [B] acetonitrile; (40:60, A:B)

flow rate

1 mL/min

pressure

1160 psi (80 bar)

column temp.

30 °C

detector

210 nm cell: 1 μL/10mm

injection

10 μL

sample

30 μg/mL (30 ppm) of Nateglinide in diluent to obtain the standard solution.