HPLC Analysis of Fluoroquinolone Antibiotics on Ascentis^® Phenyl

column

Ascentis Phenyl, 15 cm x 4.6 mm I.D., 5 μm particles (581616-U)

mobile phase

[A] 13 mM ammonium formate (pH 3.0 with concentrated formic acid): [B] acetonitrile; (80:20, A:B)

flow rate

1 mL/min, split to the MS

column temp.

35 °C

detector

MS, ESI (+) in selected ion recording (SIR) mode

injection

5 μL

sample

as indicated in 80:20, 13 mM ammonium formate (pH 3.0 with concentrated formic acid):acetonitrile