- miR-210 expression is associated with methionine-induced differentiation of trout satellite cells.
miR-210 expression is associated with methionine-induced differentiation of trout satellite cells.
The Journal of experimental biology (2017-06-04)
Mary Latimer, Nathalie Sabin, Aurélie Le Cam, Iban Seiliez, Peggy Biga, Jean-Charles Gabillard
PMID28576820
ABSTRACT
In fish, data on microRNAs (miRNAs) involved in myogenesis are scarce. In order to identify miRNAs involved in satellite cell differentiation, we used a methionine depletion/replenishment protocol to synchronize myogenic cell differentiation. Our results validated that methionine removal (72 h) from the medium strongly decreased
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Nutrient Mixture F-10 Ham, With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Sigma-Aldrich
Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose, L-glutamine, and sodium pyruvate, without sodium bicarbonate, powder, suitable for cell culture
SAFC
DMEM/Nutrient Mixture F-12 Ham, With 15 mM HEPES, without L-glutamine, L-leucine, L-lysine, L-methionine, CaCl2, MgCl2, MgSO4, sodium bicarbonate, and phenol red, powder, suitable for cell culture