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  • Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Analytical chemistry (2015-03-17)
Evgeni Eltzov, Avital Cohen, Robert S Marks
ABSTRACT

Indoor air pollution became a recent concern found to be oftentimes worse than outdoor air quality. We developed a tool that is cheap and simple and enables continuous monitoring of air toxicity. It is a biosensor with both a nondisposable (monitor) and disposable (calcium alginate pads with immobilized bacteria) elements. Various parameters to enhance its signal have been tested (including the effect of the pad's orientation, it's exposure to either temperature or time with the air toxicant analyte, and various concentrations thereof). Lastly, the sensor has demonstrated its ability to sense the presence of chemicals in a real, indoor environment. This is the first step in the creation of a sensitive and simple operative tool that may be used in different indoor environments.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Acetone, ≥99%, meets FCC analytical specifications
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Chloroform, anhydrous, ≥99%, contains 0.5-1.0% ethanol as stabilizer
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Piperidine, ≥99.5%, purified by redistillation
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Sodium chloride-35Cl, 99 atom % 35Cl
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Acetone, natural, ≥97%
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Sodium chloride, random crystals, optical grade, 99.9% trace metals basis
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Tetrahydrofuran, analytical standard
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Diethyl ether
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Tetrahydrofuran, HPLC grade, ≥99.9%, inhibitor-free
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Diethylene glycol, ReagentPlus®, 99%
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Acetone, suitable for HPLC, ≥99.9%
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Acetone, analytical standard
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Methanol, analytical standard
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(±)-Epichlorohydrin, purum, ≥99% (GC)
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N-Ethyldiisopropylamine, BASF quality, ≥98.0%
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Chloroform, analytical standard
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N-Ethyldiisopropylamine solution, suitable for peptide synthesis, ~2 M in 1-methyl-2-pyrrolidinone
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2-Mercaptoethanol, BioUltra, for molecular biology, ≥99.0% (GC)
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Triphenyl phosphite, 97%
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Piperidine, ReagentPlus®, 99%
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Tetrahydrofuran, anhydrous, ≥99.9%, inhibitor-free
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Sodium chloride, 99.999% trace metals basis
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Diethylene glycol, puriss. p.a., ≥99.0% (GC), colorless
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Tetraethyl orthosilicate, ≥99.0% (GC)
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Diethylene glycol, analytical standard
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1-Octanol, natural, ≥98%, FCC
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2-Mercaptoethanol, ≥99.0%
Supelco
Formaldehyde solution, stabilized with methanol, ~37 wt. % in H2O, certified reference material
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Sodium chloride solution, 5 M in H2O, BioReagent, for molecular biology, suitable for cell culture
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Sodium chloride, meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%