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Polyethylenimine derivate conjugated with RGD-TAT-NLS as a novel gene vector.

Polyethylenimine derivate conjugated with RGD-TAT-NLS as a novel gene vector.

Bio-medical materials and engineering (2014-09-18)

Wenfang Zhao, Kehai Liu, Shunsheng Chen, Man Man Zhu, Hui Lv, Jing Hu, Yuan Mao

ABSTRACT

To solve the contradiction between the cell toxicity and transfection efficiency of polyethylenimine (PEI) derivate in non-viral gene therapy, a novel gene vector, P123-PEI-R18 was synthesized by using biodegradable PEI derivate conjugated with trifunctional peptide RGD-TAT-NLS. The particle size of P123-PEI-R18/DNA was around 100-250 nm. The gene vector could condense DNA at the weight ratio of 2 and protect plasmid DNA from being dissolved in the blood circulation. Importantly, the complexes exhibited lower cell toxicity and higher transfection efficiency contrasted with PEI 25 kDa in vitro. P123-PEI-R18 holds high potential as a safe and efficient gene vector.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Triethylamine, ≥99.5%

Sigma-Aldrich

Bis(trichloromethyl) carbonate, purum, ≥99.0% (AT)

Sigma-Aldrich

Triethylamine, for protein sequence analysis, ampule, ≥99.5% (GC)

Sigma-Aldrich

Triethylamine, puriss. p.a., ≥99.5% (GC)

Sigma-Aldrich

Triethylamine, for amino acid analysis, ≥99.5% (GC)

Sigma-Aldrich

Triethylamine, BioUltra, ≥99.5% (GC)

Sigma-Aldrich

Rhein, technical grade

Supelco

Ethanol solution, certified reference material, 2000 μg/mL in methanol

Sigma-Aldrich

Rhein

Sigma-Aldrich

Triethylamine, ≥99%

Sigma-Aldrich

Triethylamine, ≥99.5%

Supelco

Dehydrated Alcohol, Pharmaceutical Secondary Standard; Certified Reference Material

USP

Dehydrated Alcohol, United States Pharmacopeia (USP) Reference Standard

Supelco

Triethylamine, analytical standard

Supelco

Dichloromethane solution, certified reference material, 5000 μg/mL in methanol

Supelco

Dichloromethane, Selectophore^™, ≥99.5%

Sigma-Aldrich

Ethanol, tested according to Ph. Eur.

Supelco

Dichloromethane, analytical standard

Supelco

Ethyl acetate, analytical standard

Sigma-Aldrich

N-Hydroxysuccinimide, purum, ≥97.0% (T)

Supelco

Benzene, analytical standard

Supelco

Ethanol, standard for GC

Sigma-Aldrich

Ethanol, for residue analysis

Sigma-Aldrich

Ethyl alcohol, Pure, 190 proof, ACS spectrophotometric grade, 95.0%

Sigma-Aldrich

N-Hydroxysuccinimide, 98%

Sigma-Aldrich

Dichloromethane, anhydrous, ≥99.8%, contains 40-150 ppm amylene as stabilizer

Sigma-Aldrich

Ethyl acetate, anhydrous, 99.8%

Sigma-Aldrich

Benzene, anhydrous, 99.8%

Sigma-Aldrich

Triphosgene, reagent grade, 98%

Sigma-Aldrich

Dichloromethane, suitable for HPLC, ≥99.9%, contains 40-150 ppm amylene as stabilizer