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  • Bioresolution of benzyl glycidyl ether using whole cells of Bacillus alcalophilus.

Bioresolution of benzyl glycidyl ether using whole cells of Bacillus alcalophilus.

Journal of basic microbiology (2011-11-05)
Neeraj Bala, Kirandeep Kaur, Swapandeep Singh Chimni, Harvinder Singh Saini, Shamsher Singh Kanwar
ABSTRACT

The incubation of whole Bacillus alcalophilus cells grown on a mineral supplemented medium (MSM) containing 1% (w/v) sucrose as carbon source, 1.2% (w/v) tryptone as nitrogen source at pH 6.5 and temperature 30 °C in 24 h kinetically resolved benzyl glycidyl ether (1 mg/ml) to provide (S)-benzyl glycidyl ether with 30% ee and (R)-3-benzyloxypropane-1,2-diol with 40% ee.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Benzyl glycidyl ether, 99%
Millipore
Peptone from milk solids, Refined hydrolysate
Millipore
Peptone Primatone® RL, suitable for microbiology
Sigma-Aldrich
Peptone from animal tissue, from meat, BioReagent, suitable for cell culture, suitable for plant cell culture
Millipore
Proteose Peptone, Enzymatic hydrolysate
Millipore
Peptone from animal tissue, from meat
Millipore
Peptone from casein and other animal proteins, suitable for microbiology