Skip to Content
Merck
  • Identification of critical ligand binding determinants in Mycobacterium tuberculosis adenosine-5'-phosphosulfate reductase.

Identification of critical ligand binding determinants in Mycobacterium tuberculosis adenosine-5'-phosphosulfate reductase.

Journal of medicinal chemistry (2009-08-15)
Jiyoung A Hong, Devayani P Bhave, Kate S Carroll
ABSTRACT

Mycobacterium tuberculosis adenosine-5'-phosphosulfate (APS) reductase is an iron-sulfur protein and a validated target to develop new antitubercular agents, particularly for the treatment of latent infection. To facilitate the development of potent and specific inhibitors of APS reductase, we have probed the molecular determinants that underlie binding and specificity through a series of substrate and product analogues. Our study highlights the importance of specific substitutent groups for substrate binding and provides functional evidence for ligand-specific conformational states. An active site model has been developed for M. tuberculosis APS reductase that is in accord with the results presented here as well as prior structural data reported for Pseudomonas aeruginosa APS reductase and related enzymes. This model illustrates the functional features required for the interaction of APS reductase with a ligand and provides a pharmacological roadmap for the rational design of small molecules as potential inhibitors of APS reductase present in human pathogens, including M. tuberculosis.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Adenine, ≥99%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 g
Please contact Customer Service for Availability
PLN 882.00
25 g
Please contact Customer Service for Availability
PLN 3,560.00
100 g
Please contact Customer Service for Availability
PLN 7,240.00
Sigma-Aldrich
D-(−)-Ribose, ≥99% (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 g
Please contact Customer Service for Availability
PLN 882.00
25 g
Please contact Customer Service for Availability
PLN 3,560.00
100 g
Please contact Customer Service for Availability
PLN 7,240.00
Sigma-Aldrich
D-(−)-Ribose, suitable for cell culture, BioReagent
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 g
Please contact Customer Service for Availability
PLN 882.00
25 g
Please contact Customer Service for Availability
PLN 3,560.00
100 g
Please contact Customer Service for Availability
PLN 7,240.00
Sigma-Aldrich
Adenine, BioReagent, suitable for cell culture
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 g
Please contact Customer Service for Availability
PLN 882.00
25 g
Please contact Customer Service for Availability
PLN 3,560.00
100 g
Please contact Customer Service for Availability
PLN 7,240.00
Sigma-Aldrich
Adenine, BioReagent, suitable for plant cell culture, ≥99%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 g
Please contact Customer Service for Availability
PLN 882.00
25 g
Please contact Customer Service for Availability
PLN 3,560.00
100 g
Please contact Customer Service for Availability
PLN 7,240.00
Sigma-Aldrich
Adenosine 3′-monophosphate, from yeast
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 g
Please contact Customer Service for Availability
PLN 882.00
25 g
Please contact Customer Service for Availability
PLN 3,560.00
100 g
Please contact Customer Service for Availability
PLN 7,240.00
Sigma-Aldrich
Sulfuric acid, 99.999%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 g
Please contact Customer Service for Availability
PLN 882.00
25 g
Please contact Customer Service for Availability
PLN 3,560.00
100 g
Please contact Customer Service for Availability
PLN 7,240.00
Sigma-Aldrich
D-(−)-Ribose, ≥98%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 g
Please contact Customer Service for Availability
PLN 882.00
25 g
Please contact Customer Service for Availability
PLN 3,560.00
100 g
Please contact Customer Service for Availability
PLN 7,240.00
Sigma-Aldrich
Phosphoric acid solution, NMR reference standard, 85% in D2O (99.9 atom % D), NMR tube size 4.2 mm × 8 in. , WGS-5BL Coaxial NMR tube
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 g
Please contact Customer Service for Availability
PLN 882.00
25 g
Please contact Customer Service for Availability
PLN 3,560.00
100 g
Please contact Customer Service for Availability
PLN 7,240.00
Supelco
Sulfuric acid, for the determination of nitrogen, ≥97.0%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 g
Please contact Customer Service for Availability
PLN 882.00
25 g
Please contact Customer Service for Availability
PLN 3,560.00
100 g
Please contact Customer Service for Availability
PLN 7,240.00
Sigma-Aldrich
Sulfuric acid, puriss., meets analytical specification of Ph. Eur., BP, 95-97%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 g
Please contact Customer Service for Availability
PLN 882.00
25 g
Please contact Customer Service for Availability
PLN 3,560.00
100 g
Please contact Customer Service for Availability
PLN 7,240.00
Sigma-Aldrich
D-(−)-Ribose, 98%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 g
Please contact Customer Service for Availability
PLN 882.00
25 g
Please contact Customer Service for Availability
PLN 3,560.00
100 g
Please contact Customer Service for Availability
PLN 7,240.00
Sigma-Aldrich
Sulfuric acid, ACS reagent, 95.0-98.0%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 g
Please contact Customer Service for Availability
PLN 882.00
25 g
Please contact Customer Service for Availability
PLN 3,560.00
100 g
Please contact Customer Service for Availability
PLN 7,240.00
Sigma-Aldrich
Phosphoric acid, crystalline, ≥99.999% trace metals basis
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 g
Please contact Customer Service for Availability
PLN 882.00
25 g
Please contact Customer Service for Availability
PLN 3,560.00
100 g
Please contact Customer Service for Availability
PLN 7,240.00
Sigma-Aldrich
Phosphoric acid, ≥85 wt. % in H2O, ≥99.999% trace metals basis
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 g
Please contact Customer Service for Availability
PLN 882.00
25 g
Please contact Customer Service for Availability
PLN 3,560.00
100 g
Please contact Customer Service for Availability
PLN 7,240.00
Sigma-Aldrich
Phosphoric acid, 85 wt. % in H2O, 99.99% trace metals basis
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 g
Please contact Customer Service for Availability
PLN 882.00
25 g
Please contact Customer Service for Availability
PLN 3,560.00
100 g
Please contact Customer Service for Availability
PLN 7,240.00
Sigma-Aldrich
Adenosine 5′-(α,β-methylene)diphosphate, ADP analog
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 g
Please contact Customer Service for Availability
PLN 882.00
25 g
Please contact Customer Service for Availability
PLN 3,560.00
100 g
Please contact Customer Service for Availability
PLN 7,240.00
Sigma-Aldrich
Adenosine, suitable for cell culture, BioReagent
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 g
Please contact Customer Service for Availability
PLN 882.00
25 g
Please contact Customer Service for Availability
PLN 3,560.00
100 g
Please contact Customer Service for Availability
PLN 7,240.00
Sigma-Aldrich
Adenosine, ≥99%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 g
Please contact Customer Service for Availability
PLN 882.00
25 g
Please contact Customer Service for Availability
PLN 3,560.00
100 g
Please contact Customer Service for Availability
PLN 7,240.00
Sigma-Aldrich
L-(+)-Ribose, ≥98% (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 g
Please contact Customer Service for Availability
PLN 882.00
25 g
Please contact Customer Service for Availability
PLN 3,560.00
100 g
Please contact Customer Service for Availability
PLN 7,240.00
Sigma-Aldrich
Phosphoric acid, BioReagent, suitable for insect cell culture, 85%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 g
Please contact Customer Service for Availability
PLN 882.00
25 g
Please contact Customer Service for Availability
PLN 3,560.00
100 g
Please contact Customer Service for Availability
PLN 7,240.00
Sigma-Aldrich
Phosphoric acid, BioUltra, ≥85% (T)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 g
Please contact Customer Service for Availability
PLN 882.00
25 g
Please contact Customer Service for Availability
PLN 3,560.00
100 g
Please contact Customer Service for Availability
PLN 7,240.00
Supelco
Sulfuric acid concentrate, 0.1 M H2SO4 in water (0.2N), eluent concentrate for IC
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 g
Please contact Customer Service for Availability
PLN 882.00
25 g
Please contact Customer Service for Availability
PLN 3,560.00
100 g
Please contact Customer Service for Availability
PLN 7,240.00
Sigma-Aldrich
Phosphoric acid, ACS reagent, ≥85 wt. % in H2O
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 g
Please contact Customer Service for Availability
PLN 882.00
25 g
Please contact Customer Service for Availability
PLN 3,560.00
100 g
Please contact Customer Service for Availability
PLN 7,240.00
Sigma-Aldrich
Phosphoric acid, ACS reagent, ≥85 wt. % in H2O
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
5 g
Please contact Customer Service for Availability
PLN 882.00
25 g
Please contact Customer Service for Availability
PLN 3,560.00
100 g
Please contact Customer Service for Availability
PLN 7,240.00