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T9455

Sigma-Aldrich

TMB Enhanced One Component HRP Membrane Substrate

Synonym(s):

HRP substrate, TMB, detection reagent, detection substrate

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.83

form

solution

Quality Level

200

storage temp.

2-8°C

Related Categories

General description

TMB Enhanced One Component HRP Membrane Substrate is a ready-to-use solution containing 3,3′,5,5′-tetramethylbenzidine (TMB) in a mildly acidic buffer. TMB is a substrate for horseradish peroxidase. The substrate system will react with sites on the membrane containing peroxidase, producing an insoluble permanent dark blue reaction product. To stop the reaction, rinse the membrane with reagent quality water. This substrate is not recommended for microwell or immunohistochemical applications.

Application

TMB Enhanced One Component HRP Membrane Substrate has been used as a colorimetric substrate for horseradish peroxidase (HRP) in an electrochemical enzymatic sandwich assay for the detection of interleukin-6 (IL-6) in biological fluids.

Caution

Stable at least one year at 2-8 °C.

Physical form

Ready-to-use.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Graphene Enabled Low-Noise Surface Chemistry for Multiplexed Sepsis Biomarker Detection in Whole Blood
Zupancic U, et al.
Advances in Functional Materials, 31(16), 2010638-2010638 (2021)

Protocols

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Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.

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Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

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