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SRP3272

Sigma-Aldrich

TPO mouse

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

Synonym(s):

MGDF, c-MPL Ligand

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

mouse

recombinant

expressed in E. coli

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

≥1.0 ng/mL ED50

mol wt

18.7 kDa

packaging

pkg of 10 μg

impurities

<0.1 EU/μg endotoxin, tested

color

white

UniProt accession no.

P40226

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Thpo(21832)

General description

TPO (thrombopoietin) is a hematopoietic cytokine expressed in multiple tissue types. It is predominantly produced by liver and kidney and acts as a ligand for the cell surface receptor c-mpl.

Biochem/physiol Actions

TPO (thrombopoietin) is the key regulator of thrombopoiesis for platelet lineage. In both mice and humans, inactivation of this gene results in significant decrease in number of mature platelets produced by marrow progenitors. Transgenic mice with increased expression of this protein show progressive fibrosis and osteosclerosis of the bone marrow, as well as histological changes in kidneys, leading to mesangioproliferative glomerulopathy.

Physical form

Lyophilized from 10 mM Tris, pH 8.0.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319,H335

Precautionary Statements

P261 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Opppenheim, J.J. and Feldman, M. ed.
Cytokine Reference, 295, 965-982 (2001)
Chronic thrombopoietin overexpression induces mesangioproliferative glomerulopathy in mice.
Shimoda HK
American Journal of Hematology (2007)
Transcriptional regulation of bone marrow thrombopoietin by platelet proteins.
McIntosh B and Kaushansky K
Experimental Hematology (2008)
Krasimira Rozenova et al.
Blood, 125(11), 1730-1738 (2015-02-01)
Hematopoietic stem cell (HSC) self-renewal and multilineage reconstitution are controlled by positive and negative signaling cues with perturbations leading to disease. Lnk is an essential signaling adaptor protein that dampens signaling by the cytokine thrombopoietin (Tpo) to limit HSC expansion.

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