A4471
Anti-ARTS antibody, Mouse monoclonal
clone ARTS51, purified from hybridoma cell culture
Synonym(s):
Anti-Apoptosis-Related Protein in the TGF-β Signaling Pathway, Monoclonal Anti-ARTS antibody produced in mouse
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About This Item
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biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
ARTS51, monoclonal
form
buffered aqueous solution
mol wt
antigen 32 kDa
species reactivity
human
concentration
~2 mg/mL
technique(s)
immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using a whole extract of transfected 293T (human embryonal kidney) cells expressing ARTS
isotype
IgG2b
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... SEPT4(5414)
General description
1,2 ARTS was later shown to be a key apoptotic protein mediating apoptosis initiated by other inducers.
Anti-ARTS antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the ARTS51 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic peptide corresponding to C-terminus of human ARTS protein, conjugated to KLH. ARTS (Apoptosis Related protein in the TGF-β signaling pathway), is a novel protein involved in the apoptotic pathways of transforming growth factor-β (TGF-β). The protein is derived from the human septin H5/PNUTL2/CDCrel-2a/2b gene. ARTS consists a phosphate-binding site (P-loop) death domain, which is conserved in all septins and in many different classes of ATP/GTPases. It is localized to mitochondria and translocates to the nucleus, coincident with apoptotic signals induced by TGF-β.
Apoptosis-related protein in TGF-β signaling pathway (ARTS) is a novel mitochondrial protein that mediates apoptosis induced by a variety of pro-apoptotic stimuli. Originally found to be essential for apoptosis induced by transforming growth factor-β (TGF-β) ARTS was later shown to be a key apoptotic protein mediating apoptosis initiated by other inducers. Mouse monoclonal anti-ARTS antibody recognizes an epitope within amino acids 247-266 of human ARTS molecule.
Specificity
This monoclonal anti-ARTS antibody was used to detect ARTS (Sept4_i2) protein in whole lysates of HeLa cells. HeLa ARTS knockdown (ARTS KD) stable cell line was established by using short hairpin RNAs (shRNAs) which abrogated specifically the endogenous levels of ARTS (Sept4_i2, ~30Kd)
This monoclonal Ab is the only currently commercially available antibody directed against the unique C-terminus of ARTS, that can distinguish betweenARTS ( the pro-apoptotic, tumor suppressor, specific XIAP- antagonist) and Sept4_i1 (H5,PNUTL), which is the other product of the SEPT4 gene.
This monoclonal Ab is the only currently commercially available antibody directed against the unique C-terminus of ARTS, that can distinguish betweenARTS ( the pro-apoptotic, tumor suppressor, specific XIAP- antagonist) and Sept4_i1 (H5,PNUTL), which is the other product of the SEPT4 gene.
Immunogen
synthetic peptide corresponding to C-terminal region (amino acids 247-266) of human ARTS protein, conjugated to KLH.
Application
Immunoprecipitation of COS-7 cell lysates was performed using 10 μl of monoclonal mouse anti-ARTS antibody. Antibody was allowed to bind for 4 hours on ice before precipitated using G-sepharose beads.
Monoclonal anti-ARTS antibody has been used for western blot assays. The antibody has also been used for immunostaining applications. Furthermore, the product is suitable for use in indirect ELISA microarray and immunocytochemistry assays.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Dana Mamriev et al.
Cell death & disease, 11(6), 483-483 (2020-06-27)
Many human cancers over-express B cell lymphoma 2 (Bcl-2) or X-linked inhibitor of apoptosis (IAP) proteins to evade cell death. The pro-apoptotic ARTS (Sept4_i2) protein binds directly to both Bcl-2 and XIAP and promotes apoptosis by stimulating their degradation via
Kristi L Bennett et al.
Cancer research, 69(24), 9301-9305 (2009-11-26)
Head and neck squamous cell carcinoma (HNSCC) is an aggressive cancer with low survival rates in advanced stages. To facilitate timely diagnosis and improve outcome, early detection markers (e.g., DNA methylation) are crucial for timely cancer diagnosis. In a recent
A novel mitochondrial septin-like protein, ARTS, mediates apoptosis dependent on its P-loop motif
Larisch S, et al.
Nature Cell Biology, 2(12), 915-915 (2000)
Natalia Edison et al.
Cell reports, 21(2), 442-454 (2017-10-12)
We describe a mechanism by which the anti-apoptotic B cell lymphoma 2 (Bcl-2) protein is downregulated to induce apoptosis. ARTS (Sept4_i2) is a tumor suppressor protein that promotes cell death through specifically antagonizing XIAP (X-linked inhibitor of apoptosis). ARTS and
Qian Hao et al.
Cell death & disease, 12(2), 204-204 (2021-02-26)
Apoptosis related protein in TGF-β signaling pathway (ARTS) was originally discovered in cells undergoing apoptosis in response to TGF-β, but ARTS also acts downstream of many other apoptotic stimuli. ARTS induces apoptosis by antagonizing the anti-apoptotic proteins XIAP and Bcl-2.
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