C2-BIOC

Collagenase Type II, Cls II

• Synonym(s): Collagen A
• UNSPSC Code: 12352204
• NACRES: NA.78

Properties

• form: powder
• Quality Level: 200
• storage temp.: 2-8°C

Description

General description

Collagenase Type II (Cls II) is one of the main tissue dissociation enzymes produced by Clostridium histolyticum. It aids to digest the smaller collagen fragments produced by Type I collagenase. Collagenases, a member of the matrix metalloproteinase (MMPs) family, digests collagen in the extracellular matrix (ECM). Collagens are triple helix structural proteins comprising three collagen polypeptides with high tensile strength.

This product is identical to Biochrom GmbH part numbers C2-22 and C2-28. Enzyme blending from collagenase, clostripain, with tryptic and proteolytic activities from Clostridium histolyticum. This preparation shows a high clostripain activity with the tryptic activity close to type I. Suitable for liver, bone, thyroid, heart, and salivary gland tissue. Specific activity is 125 to 250 Mandl units per milligram of powdered substance.

Application

Collagenase Type II (Cls II) has been used:

• as a component of Roswell park memorial Institute (RPMI)-1640 media to digest hepatic tissues
• to perfuse murine heart for the isolation of epicardial stromal cells (EpiSC)
• to digest collagenase and inject it into the intrarenal aorta abdominalis to perfuse the kidneys