11814320001

Formamide

=99%, liquid, pH 4-10 (20 °C, 200 g/L), suitable for hybridization

• Synonym(s): Formamide, Amide C₁, Formic amide
• Linear Formula: HCONH₂
• CAS Number: 75-12-7
• Molecular Weight: 45.04
• Beilstein: 505995
• MDL number: MFCD00007941
• UNSPSC Code: 41116100
• PubChem Substance ID: 329749563

Properties

• vapor density: 1.55 (vs air)
• vapor pressure: 0.08 mmHg ( 20 °C); 30 mmHg ( 129 °C)
• Assay: 99%
• form: liquid
• autoignition temp.: 932 °F
• mol wt: 45.04
• packaging: pkg of 500 mL
• manufacturer/tradename: Roche
• technique(s): hybridization: suitable
• color: colorless
• refractive index: n20/D 1.447 (lit.)
• pH: 4-10 (20 °C, 200 g/L)
• bp: 210 °C (lit.)
• mp: 2-3 °C (lit.)
• density: 1.134 g/mL at 25 °C (lit.)
• absorption: ≤0.08 at 290 nm
• shipped in: wet ice
• storage temp.: 2-8°C
• SMILES string: NC=O
• InChI: 1S/CH3NO/c2-1-3/h1H,(H2,2,3)
• InChI key: ZHNUHDYFZUAESO-UHFFFAOYSA-N

Description

General description

Formamide is an organic solvent, which allows for the denaturation and renaturation of nucleic acids at room temperature. This is particularly useful for protocols where reaction times are long and high temperatures would damage biological activity through chain scissions and depurination etc. Formamide reduces thermal stability of double stranded nucleic acids and is generally used for DNA renaturation or DNA-RNA hybridization. Specificity and rate of reaction are determined by formamide concentration and temperature of the reaction.

Application

Formamide has been used in:
in situ hybridization

• as a component of 2X denaturing loading dye for RNA polyacrylamide gel electrophoresis
• for the extraction of evans blue dye in hindlimb of mice in vascular leakage assay
• as a component of stop buffer used in cleavage and polyadenylation activity assays

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Quality

Contaminants: ≤5 ppm heavy metals (as Pb), ≤5 ppm Fe

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H351,H360D,H373
• Precautionary Statements: P201 - P202 - P260 - P280 - P308 + P313 - P501
• Hazard Classifications: Carc. 2 - Repr. 1B - STOT RE 2
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 1
• Flash Point(F): 305.6 °F
• Flash Point(C): 152 °C