SCT238

BioTracker Apo-15 Calcium-Independent Apoptosis Probe

• Synonym(s): Live Cell Imaging Probe
• UNSPSC Code: 12352207
• NACRES: NA.47

Properties

• mol wt: calculated mol wt 1311.33 kDa
• Quality Level: 100
• packaging: vial of 1 mg
• manufacturer/tradename: Millipore
• growth mode: N/A
• technique(s): cell culture | stem cell: suitable
• detection method: fluorometric
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

The BioTracker Apo-15 Calcium-Independent Apoptosis Probe is a calcium-independent, “turn-on” fluorescence probe emitting in the green spectrum for imaging and detection of apoptotic live cell under wash-free conditions. Like Annexin V, Apo-15 binds phosphatidylserine (PS) during early stage apoptosis when PS is translocated to the outer plasma membrane (Barth, 2020).
Apoptosis is dysregulated in numerous diseases, from inflammatory conditions to cancer. Detection of apoptosis is a key output in assessing drug efficacy, yet the low levels of free calcium in diseased tissues limit use of annexins as apoptosis detection reagents, favoring calcium-independent fluorogenic detection. Apo-15 binds negatively-charged phospholipids exposed on apoptotic cell membranes without interfering with cellular function. Apo-15 is non-fluorescent in viable cells but highly fluorescent in apoptotic cells, enabling highly specific detection of early apoptosis. Apoptotic imaging and detection with Apo-15 is broadly applicable to a wide range of cell types and experimental conditions.
Spectral Properties
Fluorescence images obtained by λex = 500 nm and emission at 520 nm.
Reference
Barth, Nicole D., et al. "A fluorogenic cyclic peptide for imaging and quantification of drug-induced apoptosis." Nature communications 11.1 (2020): 1-14.

Application

Purity > 98% confirmed by HPLC. Identification confirmed by H NMR, LC-MS, and elemental analysis.

Features and Benefits

Green fluorescent probe for detecting early stage apoptosis in live cells by microscopy or flow cytometry.

Physical form

Lyophilized. Orange solid.

Storage and Stability

Store at -20°C, desiccate and protect from light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening

Other Notes

Near-Infrared probe for detection and visualization of myeloperoxidase (MPO) activity in vitro and in vivo.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable