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T2319

Sigma-Aldrich

Trizma® hydrochloride solution

pH 7.5, BioPerformance Certified, 1 M, suitable for cell culture

Synonym(s):

Tris hydrochloride solution

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100 ML
£42.00
1 L
£85.30

£42.00

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100 ML
£42.00
1 L
£85.30

About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

£42.00

Please contact Customer Service for Availability
Request a Bulk Order

grade

BioPerformance Certified
for molecular biology

Quality Level

200

form

solution

concentration

1 M

technique(s)

cell culture | mammalian: suitable

impurities

DNase, RNase, Protease, none detected
 bioburden, tested
 endotoxin, tested
≤5 ppm Heavy metals (as Pb)

pH

7.5

useful pH range

7.0-9.0

absorption

≤0.05 at 290 at 40%

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General description

A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.

Application

Trizma®hydrochloride solution has been used in Tween tris-buffered saline solution.[1] It has also been used in the preparation of streptavidin-alkaline phosphatase used for enzyme linked immunosorbent assay (ELISA).[2]

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SLCR5430
SLCR5430
0000287426
0000287428
0000287426

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The development of a sensitive and specific ELISA for mouse eosinophil peroxidase: assessment of eosinophil degranulation ex vivo and in models of human disease
Ochkur SI, et al.
Journal of Immunological Methods, 375(1-2), 138-147 (2012)
Genomic maps of long noncoding RNA occupancy reveal principles of RNA-chromatin interactions.
Ci C Chu, Kun K Qu, Franklin L FL Zhong, Steven E SE Artandi, Howard Y HY Chang
Molecular Cell, 44, 667-678 null
An in vitro assay for sonothrombolysis based on the spectrophotometric measurement of clot thickness
Wang Z, et al.
Journal of Ultrasound in Medicine : Official Journal of the American Institute of Ultrasound in Medicine, 36(4), 681-698 (2017)
Daniela Amann-Zalcenstein et al.
Nature immunology, 21(12), 1574-1584 (2020-10-21)
A classical view of blood cell development is that multipotent hematopoietic stem and progenitor cells (HSPCs) become lineage-restricted at defined stages. Lin-c-Kit+Sca-1+Flt3+ cells, termed lymphoid-primed multipotent progenitors (LMPPs), have lost megakaryocyte and erythroid potential but are heterogeneous in their fate.
Zuojun Wang et al.
Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine, 36(4), 681-698 (2017-02-06)
For improved thrombolysis therapy based on ultrasound irradiation, researchers and practitioners would strongly benefit from an easy and efficient in vitro assay system of thrombolysis activity involving irradiated ultrasound. For the present study, we designed a new in vitro sonothrombolysis
View All Related Papers

Questions

1–2 of 2 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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