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  • Tristetraprolin functions in cytoskeletal organization during mouse oocyte maturation.

Tristetraprolin functions in cytoskeletal organization during mouse oocyte maturation.

Oncotarget (2016-07-28)
Xiaohui Liu, Xiaoyan Li, Rujun Ma, Bo Xiong, Shao-Chen Sun, Honglin Liu, Ling Gu
ABSTRACT

Tristetraprolin (TTP), a member of TIS11 family containing CCCH tandem zinc finger, is one of the best characterized RNA-binding proteins. However, to date, the role of TTP in mammalian oocytes remains completely unknown. In the present study, we report the altered maturational progression and cytokinesis, upon specific knockdown of TTP in mouse oocytes. Furthermore, by confocal scanning, we observe the failure to form cortical actin cap during meiosis of TTP-depleted oocytes. Loss of TTP in oocytes also results in disruption of meiotic spindle morphology and chromosome alignment. In support of these findings, incidence of aneuploidy is accordingly increased when TTP is abated in oocytes. Our results suggest that TTP as a novel cytoskeletal regulator is required for spindle morphology/chromosome alignment and actin polymerization in oocytes.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Monoclonal Anti-β-Actin antibody produced in mouse, clone AC-15, ascites fluid
Sigma-Aldrich
Anti-Tristetraprolin Antibody, from rabbit, purified by affinity chromatography