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L4146

Sigma-Aldrich

Leptin human

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder

Synonym(s):

OB

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
51111800
NACRES:
NA.32

recombinant

expressed in E. coli

Quality Level

sterility

sterile; 0.2 μm filtered

Assay

≥97% (SDS-PAGE)

form

lyophilized powder

potency

≤3.0 ng/mL ED50/EC50

mol wt

predicted mol wt ~16 kDa

impurities

≤1.0 EU/μg endotoxin

UniProt accession no.

shipped in

ambient

storage temp.

−20°C

Gene Information

human ... LEP(3952)

General description

The gene LEP (leptin) is mapped to human chromosome 7q31. It is a 16kDa peptide. Leptin is released in the circulation and goes to the CNS (central nervous system). In CNS, it works by associating with leptin receptor.

Application

Leptin human has been used:
  • to study the effect of leptin on ACAT1 (acyl-coenzyme A:cholesterol acyltransferase 1) expression and cholesterol efflux from primary cultured human macrophages
  • to study the effect of leptin on expression of IGFBP2 (insulin growth factor binding protein 2) in skeletal muscle
  • to study the effect of leptin on HLA-G (human leukocyte antigen G) expression in placental trophoblasts
  • to study the effect of leptin on in vitro maturation, fertilization and development of horse oocytes after ICSI (intracytoplasmic sperm injection)

Biochem/physiol Actions

Leptin is a hormone produced primarily in adipocytes, although leptin mRNA has also been identified in placenta and fetal tissues, gastric tissue and liver. Its primary site of action appears to be on neurons in the hypothalamus that are involved in regulating energy balance, appetite, and body weight. Leptin increases the production of nitric oxide in endothelial cells and stimulates angiogenesis in vitro and in vivo.
Human and mouse leptin share ~84% sequence identity.
Leptin is believed to play a role in normal pregnancy development.
Mutations in LEP (leptin) are linked with type-2 diabetes. Presence of high levels of leptin in plasma is related to adiposity.

Physical form

Lyophilized from a 0.2 μm filtered solution with 0.0045 mM NaHCO3

Analysis Note

The biological activity is measured by its ability to induce proliferation of leptin-dependent human OB-R transfected murine BAF3 cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Shih-Ping Cheng et al.
Oncology reports, 23(6), 1721-1727 (2010-04-30)
Excess body weight is associated with a moderately increased risk of thyroid cancer. Adipocyte-derived hormone, leptin, has been shown to enhance cell growth and migration in many cancer types. Limited evidence suggests that leptin has direct actions on the thyroid
Eiji Kashiwagi et al.
American journal of translational research, 12(6), 3033-3045 (2020-07-14)
Adipocytokines such as leptin and adiponectin have functions in metabolism as well as the development and progression of various types of malignancies. However, little is known about their role in bladder cancer. In this study, we investigated whether leptin, adiponectin
Elisabetta Trevellin et al.
Oncotarget, 6(13), 11203-11215 (2015-04-11)
Obesity is associated with cancer risk in esophageal adenocarcinoma (EAC). Adipose tissue directly stimulates tumor progression independently from body mass index (BMI), but the mechanisms are not fully understood. We studied the morphological, histological and molecular characteristics of peritumoral and
Masafumi Kurajoh et al.
Cardiovascular diabetology, 14, 117-117 (2015-09-05)
It has been shown that visceral fat accumulation is associated with autonomic dysfunction, though the precise mechanism remains unclear. A recent basic study found that leptin can directly modulate autonomic function through the dorsomedial hypothalamus in relation to obesity. Here
L Pérusse et al.
Obesity research, 9(2), 135-169 (2001-04-24)
This report constitutes the seventh update of the human obesity gene map incorporating published results up to the end of October 2000. Evidence from the rodent and human obesity cases caused by single-gene mutations, Mendelian disorders exhibiting obesity as a

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