70148

Nutrient Agar

suitable for microbiology, NutriSelect^® Plus

• Synonym(s): NA, standard nutrient agar
• UNSPSC Code: 41171605
• NACRES: NA.74

Properties

• Agency: according to ISO 16266:2006
• Quality Level: 200
• sterility: non-sterile
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 15 g/L ; meat extract, 1 g/L ; peptone, 5 g/L ; sodium chloride, 5 g/L ; yeast extract, 2 g/L
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• final pH: 7.1±0.2 (25 °C)
• application(s): agriculture; clinical testing; environmental; food and beverages; life science and biopharma; water monitoring; microbiology
• storage temp.: 2-25°C
• suitability: nonselective for Aspergillus spp.; nonselective for Bacillus spp.; nonselective for Candida spp.; nonselective for Enterococcus spp.; nonselective for Escherichia coli; nonselective for Listeria spp.; nonselective for Pseudomonas spp.; nonselective for Staphylococcus spp.; nonselective for Streptococcus spp.; nonselective for bacteria (General Media); nonselective for coliforms

Description

General description

Nutrient medium is a general basic culture medium for less fastidious microorganisms. Meat extract, yeast extract and peptone provide nitrogen, vitamins, amino acids and carbon for growth. Sodium Chloride ensure osmotic balance. Thus, when adding blood, as supplement the blood cells will not rupture. Addition of 10% different biological fluids such as blood, serum and egg yolk makes it suitable for the cultivation of related fastidious organism. Acc. to Gray et al. the addition of 0.05% potassium tellurite makes the Nutrient medium an excellent enrichment medium for Listeria monocytogenes.

Application

• Nutrient Agar is used for the routine cultivation of microorganisms not requiring specific nutritional requirements
• It is also used for the enumeration and enrichment of bacteria
• Nutrient Agar can be used as a base for preparing special culture media.

Preparation Note

Suspend 28 g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes.

Other Notes

Studies on heterotrophic bacteria in some Argentine soils

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves