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567569

Sigma-Aldrich

Spautin-1

a quinazolin compound that acts as a specific and potent inhibitor of autophagy

Synonym(s):

Spautin-1, Autophagy Inhibitor II, C43, 6-fluoro-N-[4-fluorobenzyl]quinazolin-4-amine, Specific and Potent AUtophagy Inhibitor 1, C43, 6-fluoro-N-[4-fluorobenzyl]quinazolin-4-amine, Specific and Potent AUtophagy Inhibitor 1, Autophagy Inhibitor II

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About This Item

Empirical Formula (Hill Notation):
C15H11F2N3
Molecular Weight:
271.26
MDL number:
MFCD16962292
UNSPSC Code:
12352200
NACRES:
NA.77

Quality Level

100

Assay

≥99% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

off-white

solubility

DMSO: 50 mg/mL, clear, colorless

shipped in

ambient

storage temp.

2-8°C

General description

A quinazolin compound that acts as a specific and potent inhibitor of autophagy and promotes Vps34 PI 3-kinase complex degradation by blocking the activity of USP 10 (IC50 = 580 nM) and USP 13 (IC50 = 690 nM) deubiquitinating enzymes. However, it does not affect the lipid kinase activity of Vps34. Also shown to reduce the level of cytoplasmic and nuclear p53. Sensitizes breast cancer cell lines (BCAP37, MCF-7, and BT549) to apoptosis under conditions of nutritional deprivation. Also shown to inhibit etoposide-induced cell death in Bax/Bak double knockout (DKO) cells. Normal cell lines (e.g., MDCK cells) do not undergo autophagy in the presence of Spautin-1.

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Lui, J., et al. 2011, Cell.147, 223.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

CorrosionExclamation mark
GHS05,GHS07

Signal Word

Danger

Hazard Statements

H302,H318

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Maxwell Eisenbaum et al.
Glia, 72(1), 184-205 (2023-09-05)
Tauopathies are a collection of neurodegenerative diseases characterized by the accumulation of pathogenic aggregates of the microtubule-associated protein tau. Despite the prevalence and diversity of tau astrogliopathy in tauopathies, the interactions between astrocytes and tau in the brain, and the
Javier Chicote et al.
Frontiers in pharmacology, 11, 580343-580343 (2020-11-13)
Macroautophagy (hereafter autophagy) is a multistep intracellular catabolic process with pleiotropic implications in cell fate. Attending to its activation, autophagy can be classified into inducible or constitutive. Constitutive, or basal autophagy, unfolds under nutrient-replete conditions to maintain the cellular homeostasis.

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