Antifungals Reagents in Cell Culture

• Fungal Contamination in Cell Culture
• Antifungals for Cell Culture
• Amphotericin B
• Nystatin
• Other Antifungals for Cell Culture
• Other Sources of Contamination

Fungal Contamination in Cell Culture

Fungal infections in cell cultures can have severe consequences for cell health and experimental reproducibility. Fortunately, these infections are often visible to the naked eye. Fungal contaminations change the color of phenol red and can cause rapid on-set of turbidity in the cell culture media. While testing cultures for fungi is an important part of routine quality control, it is important to eliminate and prevent fungal infections before they start.

Antifungals for Cell Culture

Antifungal compounds are chemicals that target the integrity of fungal cell wall or cell membrane, thereby controlling or eliminating fungal infections in cell cultures. Most antifungals either directly or indirectly increase the porosity of the fungi cell wall or membrane. These compounds can also interfere with the steps in cell wall synthesis. In addition to antibacterials, antifungals have been evolved as critical additives to culture medium when culturing cell lines.

Amphotericin B

Amphotericin B is also known as Fungizone and is most effective against fungi and yeast infections. The compound binds to sterols and induces formation of pores in the membrane. These pores allow small molecules to leak out, thereby destroying the infection. Amphotericins are stable in cell culture for 3 days at 37˚C.

Product #	Name	Form	Recommended Concentration
E3789	Amphotericin B low endotoxin	Powder	2.5 mg/mL
A2411	Amphotericin B from Streptomyces sp.	Solid	2.5 mg/mL
A9528	Amphotericin B solubilized	Solubilized powder	5.6 mg/mL
A2942	Amphotericin B solution	Liquid	10 ml/L

Nystatin

The antifungal Nystatin is also known as Mycostatin or Fungicidine. Like Amphotericin B, Nystatin eliminates fungal infections by binding to the sterols in the fungal cell membrane and causing small molecules to leak out. However, compared to Amphotericin B, Nystatin has a larger concentration window and is less toxic to the cells in culture. Nystatins are stable in cell culture for 3 days at 37 ˚C.

We also offer water-soluble versions of Nystatin (N6803 & N9150) which are convenient to use, avoid the use of organic solvents, and are most suitable to protect sensitive cells against contamination. 

Product #	Name	Form	Recommended Concentration
N6803	Nystatin water soluble	Solubilized powder	3-5 mg/mL
N9150	Nystatin Ready made solution	Liquid	3-5 mg/mL
N6261	Nystatin	Powder	2.5 mg/mL
N4014	Nystatin	Powder	50 mg/mL
N1638	Nystatin Suspension	Suspension	24 ml/L

Other Antifungals for Cell Culture

Product #	Name	Form
A5955	Antibiotic Antimycotic Solution (100×), Stabilized	Suspension
T5535	Thiabendazole for plant cell culture	Powder
P8556	Pentachloronitrobenzene for plant cell culture	Powder
H3274	Hygromycin B (for insect cell culture)	Powder
H9773	Hygromycin B (for plant cell culture)	Powder
B8416	Bleomycin sulfate from Streptomyces verticillus	Solid

Other Sources of Contamination

Contamination is a significant concern in cell culture experiments and gathering reliable data. Mycoplasma contamination, bacterial contamination, and other common contaminants can negatively affect data reproducibility. Therefore, it is important to protect your cultures by exploring antibiotic powders and solutions for your cells, selecting the right antibiotics for your cells, and determining a suitable antibiotic concentration for your cells.