HPLC Analysis of Protein Standards (1) on BIOshell™ A400 Protein C4
Conditions | |
|---|---|
| column | BIOshell A400 Protein C4, 10 cm X 2.1 mm, 3.4 µm particles (66825-U) |
| mobile phase | (A) 75:25, [0.1% TFA in water]:[0.1% TFA in acetonitrile] ; (B) 25:75, [0.1% TFA in water]:[0.1% TFA in acetonitrile] |
| gradient | 25 to 65% B in 8 min |
| flow rate | 0.3 mL/min |
| 0.3 mL/min | 1430 psi (99 bar) |
| sample | protein mixture, g/L ea: lysozyme, 0.1; haptoglobulin, 0.3; conalbumin, 0.3; trypsin inhibitor, 0.3; glutamate dehydrogenase, 0.8; beta-galactosidase, 0.7; ovalbumin, 0.8; lactate dehydrogenase, 0.4 |
| injection | 1 µL |
| detector | UV, 215 nm |
Description | |
|---|---|
| Analysis Note | This application demonstrates the utility of the BIOshell A400 Protein C4 column for the reversed-phase chromatography of proteins from a variety of sources and of different molecular weights. |
| Categories | Analytical Chromatography, Proteins |
| Featured Industry | Life Science and Biopharma |
| suitability | application for HPLC |
Materials
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