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UHPLC/MS/MS Analysis of 25 Small Molecule Pharmaceutical Compounds on Ascentis® Express RP-Amide

UHPLC/MS/MS Analysis of 25 Small Molecule Pharmaceutical Compounds on Ascentis® Express RP-Amide application for UHPLC-MS

Matériaux

Colonne analytique

Réf. du produit
Description
Tarif

53913-U

Ascentis® Express RP-Amide, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

Composant de phase mobile

Réf. du produit
Description
Tarif

A1542

Acétate d′ammonium

for molecular biology, ≥98%

Étalon

Réf. du produit
Description
Tarif

37894

Imidacloprid

PESTANAL®, analytical standard

254819

Hydroquinidine hydrochloride

98%

442641

Methapyrilene HCl

analytical standard

31598

4-aminobiphényl

analytical standard

1463508

Nifedipine

United States Pharmacopeia (USP) Reference Standard

1444279

Mirtazapine

United States Pharmacopeia (USP) Reference Standard

1134379

Clarithromycin

United States Pharmacopeia (USP) Reference Standard

1370462

Losartan Potassium

United States Pharmacopeia (USP) Reference Standard

1457902

Nefazodone hydrochloride

United States Pharmacopeia (USP) Reference Standard

90935

Atrazine

certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

B-004

Benzoylecgonine solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

1019701

Amiloride hydrochloride

United States Pharmacopeia (USP) Reference Standard

1314009

Hydrochlorothiazide

United States Pharmacopeia (USP) Reference Standard

1367005

Lincomycin hydrochloride

United States Pharmacopeia (USP) Reference Standard

1370270

Loratadine

United States Pharmacopeia (USP) Reference Standard

A-923

Amitriptyline hydrochloride solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

H-030

Haloperidol solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

I-902

Imipramine hydrochloride solution

1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

M-919

Mianserin hydrochloride solution

1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

1078802

Buspirone hydrochloride

United States Pharmacopeia (USP) Reference Standard

1393005

Mesoridazine besylate

United States Pharmacopeia (USP) Reference Standard

1467804

Nizatidine

United States Pharmacopeia (USP) Reference Standard

1460703

Nevirapine anhydrous

United States Pharmacopeia (USP) Reference Standard

1338313

Imiquimod

United States Pharmacopeia (USP) Reference Standard

CONDITIONS

column

Ascentis Express RP-Amide, 10 cm x 2.1 mm I.D., 2.7 μm particles (53913-U)

mobile phase

[A] 0.1% acetic acid, 5 mM ammonium acetate in water; [B] 5 mM ammonium acetate in acetonitrile

gradient

5% B for 1 min; to 43% B in 6 min; held for 1 min; to 80% B in 4 min; held for 1 min; to 5% B in 1 min; held for 3 min

flow rate

0.4 mL/min

detector

MS, ESI(+) MRM

injection

1 μL

Description

Application

This application highlights the advantage of an embedded polar group (EPG) HPLC phase for the separation of various small molecules. The use of an Ascentis® RP-Amide column allowed for the development of a single UPLC-MS/MS method for the analysis of 25 pharmaceuticals that varied significantly in both chemical and physical properties (log P, pKa, etc.). The presence of an embedded amide group allowed for retention of more polar analytes in comparison to a traditional C18 phase.

Informations légales

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS