HPLC Analysis of Purines and Pyrimidines of Nucleic Acids on Ascentis^® Si (Silica)

column

Ascentis Si, 10 cm × 2.1 mm I.D., 5 μm particles (581508-U)

mobile phase

[A] 200 mM ammonium formate (pH 3.0, with formic acid): [B] acetonitrile; (10:90, A:B)

flow rate

0.2 mL/min

column temp.

35 °C

detector

UV, 270 nm

injection

1 μL

sample

100 μg/mL in mobile phase