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HPLC Analysis of Fusarium Mycotoxins on Ascentis® Express Phenyl-Hexyl

HPLC Analysis of Fusarium Mycotoxins on Ascentis® Express Phenyl-Hexyl application for HPLC

Matériaux

Colonne analytique

Réf. du produit
Description
Tarif

Ascentis® Express Phenyl-Hexyl, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

Étalon

Réf. du produit
Description
Tarif

Nivalenol solution

100 μg/mL in acetonitrile, analytical standard

15-Acetyldeoxynivalenol

reference material

3-Acetyldeoxynivalenol from Fusarium roseum

CONDITIONS

column

Ascentis Express Phenyl-Hexyl, 10 cm x 2.1 mm I.D., 2.7 μm particles (53336-U)

mobile phase

[A] water, acetonitrile, 90:10; [B] acetonitrile

gradient

0% B held for 0.5 min; to 15% B in 1 min; held at 15% B for 6 min

flow rate

0.3 mL/min

pressure

192 bar (2780 psi)

column temp.

30 °C

detector

UV, 218 nm

injection

10 μL

sample

5 mg/L ea. in 95:5, water:acetonitrile

Description

Remarque sur l'analyse

The trichothecene vomitoxins of Fusarium are significant plant pathogens of grain and forage crops that can lead to anorexic effects with livestock. This application demonstrates resolution of deoxynivalenol and three of its analogs.

Informations légales

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany