Fluorochinolons

column

Discovery C18, 15 cm × 4.6 mm I.D., 5 μm particles (504955)

mobile phase

[A] water (10 mM ammonium acetate, pH 3.0 with acetic acid); [B] acetonitrile; (85:15, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

UV, 254 nm

injection

10 μL

sample

50 μg/mL each in mobile phase