HPLC Analysis of 3,5-tBu-MeOBIPHEP Enantiomers on Astec^® CHIROBIOTIC^® V

column

CHIROBIOTIC V, 25 cm x 4.6 mm I.D., 5 μm particles (11024AST)

mobile phase

[A] n-hexane; [B] 10% ethanol in n-hexane; (75:25, A:B)

flow rate

1 mL/min

column temp.

18 °C

detector

UV, 230 nm

sample

3,5-tBu-MeOBIPHEP ((6,6′-dimethoxybiphenyl-2,2′-diyl)bis[bis(3,5-di-tert-butylphenyl)phosphine]), 1 mg/mL in ethanol with 5 drops of H₂O₂ added for oxidation