Ratiometric Fluorescence Detection of Phosphorylated Amino Acids Through Excited-State Proton Transfer by Using Molecularly Imprinted Polymer (MIP) Recognition Nanolayers.

Ratiometric Fluorescence Detection of Phosphorylated Amino Acids Through Excited-State Proton Transfer by Using Molecularly Imprinted Polymer (MIP) Recognition Nanolayers.

Chemistry (Weinheim an der Bergstrasse, Germany) (2017-09-05)

Wei Wan, Ana B Descalzo, Sudhirkumar Shinde, Hardy Weißhoff, Guillermo Orellana, Börje Sellergren, Knut Rurack

RÉSUMÉ

A 2,3-diaminophenazine bis-urea fluorescent probe monomer (1) was developed. It responds to phenylphosphate and phosphorylated amino acids in a ratiometric fashion with enhanced fluorescence accompanied by the development of a redshifted emission band arising from an excited-state proton transfer (ESPT) process in the hydrogen-bonded probe/analyte complex. The two urea groups of 1 form a cleft-like binding pocket (K

MATÉRIAUX

Référence du produit

Marque

Description du produit

477060

Sigma-Aldrich

2-Isocyanatoethyl methacrylate, contains ≤0.1% BHT as inhibitor, 98%

409448

Sigma-Aldrich

Benzyl methacrylate, 96%, contains monomethyl ether hydroquinone as inhibitor

661376

Sigma-Aldrich

2,3-Diaminophenazine, 90%

B97208

Sigma-Aldrich

2-tert-Butyl-4-methylphenol, 99%