Accéder au contenu
Merck

miR-330-3p controls cell proliferation by targeting early growth response 2 in non-small-cell lung cancer.

Acta biochimica et biophysica Sinica (2015-05-04)
Xuzhi Liu, Hanbing Shi, Bo Liu, Jianing Li, Yaxin Liu, Baiquan Yu
RÉSUMÉ

Non-small-cell lung cancer (NSCLC) is one of the most common lung cancers, and microRNAs (miRNAs) have been reported to play essential roles in NSCLC. Recent studies have indicated that miR-330-3p expression is up-regulated in NSCLC samples and in tissues of NSCLC brain metastasis. In this study, up-regulation of miR-330-3p expression was confirmed in NSCLC and 20 NSCLC patient samples. Furthermore, miR-330-3p was over-expressed in NSCLC cell lines A549 and H23, and the promotive function of miR-330-3p was investigated in regulating NSCLC cell proliferation and cell cycle distribution. To identify potential target genes of miR-330-3p in NSCLC, the miRNA target prediction databases were used. Luciferase activity assay and real-time RT-PCR analysis confirmed that miR-330-3p is negatively correlated with the expression of early growth response 2 (EGR2). Moreover, it was also found that EGR2 mRNA contains two potential binding sites for miR-330-3p. Knock-down of EGR2 with siRNA was demonstrated to have a similar effect as the over-expression of miR-330-3p in NSCLC cell lines. Taken together, our results show that EGR2 is a target of miR-330-3p.

MATÉRIAUX
Référence du produit
Marque
Description du produit

Sigma-Aldrich
Diméthylsulfoxyde, Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
Sigma-Aldrich
Diméthylsulfoxyde, for molecular biology
Sigma-Aldrich
Diméthylsulfoxyde, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
Sigma-Aldrich
Sodium Dodecyl Sulfate, BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)
Sigma-Aldrich
Diméthylsulfoxyde, ≥99.5% (GC), suitable for plant cell culture
Sigma-Aldrich
Sodium Dodecyl Sulfate, ≥99.0% (GC), dust-free pellets
Sigma-Aldrich
Bleu de thiazol (thiazolyl blue tetrazolium bromide), 98%
Sigma-Aldrich
Bleu de thiazol (thiazolyl blue tetrazolium bromide), powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)
Sigma-Aldrich
Sodium Dodecyl Sulfate solution, BioUltra, for molecular biology, 10% in H2O
Sigma-Aldrich
Diméthylsulfoxyde, anhydrous, ≥99.9%
Sigma-Aldrich
Diméthylsulfoxyde, BioUltra, for molecular biology, ≥99.5% (GC)
Sigma-Aldrich
Sodium Dodecyl Sulfate solution, BioUltra, for molecular biology, 20% in H2O
Sigma-Aldrich
Sodium Dodecyl Sulfate, ACS reagent, ≥99.0%
Sigma-Aldrich
Sodium Dodecyl Sulfate, BioUltra, for molecular biology, ≥99.0% (GC)
Sigma-Aldrich
Sodium Dodecyl Sulfate, ReagentPlus®, ≥98.5% (GC)
Supelco
Sodium Dodecyl Sulfate, dust-free pellets, suitable for electrophoresis, for molecular biology, ≥99.0% (GC)
Sigma-Aldrich
Diméthylsulfoxyde, PCR Reagent
Sigma-Aldrich
Sodium Dodecyl Sulfate, ≥98.0% (GC)
Sigma-Aldrich
Diméthylsulfoxyde, meets EP testing specifications, meets USP testing specifications
Sigma-Aldrich
Sodium Dodecyl Sulfate, BioXtra, ≥99.0% (GC)
Sigma-Aldrich
Sodium Dodecyl Sulfate, 92.5-100.5% based on total alkyl sulfate content basis
Sigma-Aldrich
Sodium Dodecyl Sulfate, tested according to NF, mixture of sodium alkyl sulfates consisting mainly of sodium dodecyl sulfate
Sigma-Aldrich
Sodium Dodecyl Sulfate, ≥90% ((Assay))
Sigma-Aldrich
Sodium Dodecyl Sulfate, BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC), free-flowing, Redi-Dri
Sigma-Aldrich
MISSION® esiRNA, targeting human EGR2
Sigma-Aldrich
MISSION® esiRNA, targeting mouse Egr2