A novel functional role for MMSET in RNA processing based on the link between the REIIBP isoform and its interaction with the SMN complex.

A novel functional role for MMSET in RNA processing based on the link between the REIIBP isoform and its interaction with the SMN complex.

PloS one (2014-06-14)

Fabio Mirabella, Alexander Murison, Lauren I Aronson, Christopher P Wardell, Andrew J Thompson, Sarah J Hanrahan, Jacqueline H L Fok, Charlotte Pawlyn, Martin F Kaiser, Brian A Walker, Faith E Davies, Gareth J Morgan

PMID24923560

RÉSUMÉ

The chromosomal translocation t(4;14) deregulates MMSET (WHSC1/NSD2) expression and is a poor prognostic factor in multiple myeloma (MM). MMSET encodes two major protein isoforms. We have characterized the role of the shorter isoform (REIIBP) in myeloma cells and identified a clear and novel interaction of REIIBP with members of the SMN (survival of motor neuron) complex that directly affects the assembly of the spliceosomal ribonucleic particles. Using RNA-seq we show that REIIBP influences the RNA splicing pattern of the cell. This new discovery provides novel insights into the understanding of MM pathology, and potential new leads for therapeutic targeting.

MATÉRIAUX

Référence du produit

Marque

Description du produit

F8775

Sigma-Aldrich

Formaldéhyde solution, 36.5-38% in H₂O

S3014

Sigma-Aldrich

Chlorure de sodium, DNase, RNase, and protease, none detected, ≥99% (titration)

F1635

SAFC

Formaldéhyde solution, contains 10-15% methanol as stabilizer, 37 wt. % in H₂O

S8776

Sigma-Aldrich

Chlorure de sodium solution, 0.9% in water, BioXtra, suitable for cell culture

S5886

Sigma-Aldrich

Chlorure de sodium, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%

S5150

Sigma-Aldrich

Chlorure de sodium solution, 5 M in H₂O, BioReagent, suitable for cell culture

59222C

SAFC

Chlorure de sodium solution, 5 M

47608

Sigma-Aldrich

Formaldéhyde solution, BioReagent, ≥36.0% in H₂O (T)

71386

Sigma-Aldrich

Chlorure de sodium solution, BioUltra, ~5 M in H₂O

204439

Sigma-Aldrich

Chlorure de sodium, 99.999% trace metals basis

71376

Sigma-Aldrich

Chlorure de sodium, BioUltra, ≥99.5% (AT)

47083-U

Supelco

Formaldéhyde solution, stabilized with methanol, ~37 wt. % in H₂O, certified reference material

252549

Sigma-Aldrich

Formaldéhyde solution, ACS reagent, 37 wt. % in H₂O, contains 10-15% Methanol as stabilizer (to prevent polymerization)

S7653

Sigma-Aldrich

Chlorure de sodium, BioXtra, ≥99.5% (AT)

A7502

Sigma-Aldrich

DL-Alanine, ≥99% (HPLC)

S6191

Sigma-Aldrich

Chlorure de sodium, BioPerformance Certified, ≥99% (titration), suitable for insect cell culture, suitable for plant cell culture

450006

Sigma-Aldrich

Chlorure de sodium, AnhydroBeads^™, −10 mesh, 99.999% trace metals basis

15512

Sigma-Aldrich

Formaldéhyde solution, meets analytical specification of USP, ≥34.5 wt. %

451940

Sigma-Aldrich

Sodium chloride-³⁵Cl, 99 atom % ³⁵Cl

S1679

Sigma-Aldrich

Chlorure de sodium, meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%

S6546

Sigma-Aldrich

Chlorure de sodium solution, 5 M

378860

Sigma-Aldrich

Chlorure de sodium, random crystals, optical grade, 99.9% trace metals basis

47673

Sigma-Aldrich

Formaldéhyde solution, tested according to Ph. Eur.

W381810

Sigma-Aldrich

DL-Alanine, ≥99%, FCC, FG

S0817

Sigma-Aldrich

Chlorure de sodium solution, 0.85%

606758

Sigma-Aldrich

Formaldehyde-¹²C solution, 20% in H₂O, 99.9 atom % ¹²C

71387

Supelco

Chlorure de sodium, reference material for titrimetry, certified by BAM, >99.5%

PHR1321

Supelco

Chlorure de sodium, Pharmaceutical Secondary Standard; Certified Reference Material

A0325000

Alanine, European Pharmacopoeia (EP) Reference Standard

S6150

Sigma-Aldrich

Chlorure de sodium, tablet