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The role of anti-elastin antibodies in a mouse model of asthma.

Asian Pacific journal of allergy and immunology (2021-09-21)
Tomoshige Wakayama, Kumiya Sugiyama, Shingo Tokita, Hajime Arifuku, Naotatsu Otsuji, Kei Sugitate, Takayoshi Owada, Kenya Koyama, Hirokuni Hirata, Masafumi Arima, Yasutsugu Fukushima
RÉSUMÉ

The role of anti-elastin antibody (Ab) in the lung is unclear, although they may be involved in chronic obstructive pulmonary disease (COPD). Recently, increased anti-elastin Ab levels were reported in asthma. To elucidate the role of anti-elastin Ab in asthma, we created a murine asthma model. Anti-elastin Ab in the airway was neutralized by intratracheal administration of elastin peptide, and the inhibitory effects of anti-elastin Ab on airway remodeling were evaluated. BALB/c mice were immunized with ovalbumin (OVA) on days 0 and 14. After immunization, the mice received booster OVA via inhalation twice per week for 9 weeks, and bronchoalveolar lavage fluid (BALF) and lung tissues were evaluated. In lung tissues, airway remodeling occurred after 9 weeks of OVA sensitization. Peak levels of anti-elastin Ab and eosinophils in BALF were detected after 3 weeks of OVA sensitization. Anti-elastin Ab and eosinophil levels in BALF were significantly reduced after 3 weeks by the neutralization of anti-elastin Ab. Peak transforming growth factor-β1 levels in BALF were detected at 3 weeks after OVA sensitization and were significantly reduced by the neutralization of anti-elastin Ab. Airway remodeling in lung tissues was also significantly inhibited by the neutralization of anti-elastin Ab. In our murine asthma model, anti-elastin Ab was recruited to the airway by OVA-induced allergic inflammation. Airway remodeling was inhibited by the neutralization of anti-elastin Ab. Anti-elastin Ab may contribute to the progression of airway remodeling.

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Sigma-Aldrich
Anti-Mouse IgG F(ab′)2, F(ab′)2 fragment, highly cross absorbed-Peroxidase antibody produced in goat, affinity isolated antibody, lyophilized powder