- Protocol for the Bottom-Up Proteomic Analysis of Mouse Spleen.
Protocol for the Bottom-Up Proteomic Analysis of Mouse Spleen.
STAR protocols (2020-12-31)
Paul Dowling, Stephen Gargan, Margit Zweyer, Michael Henry, Paula Meleady, Dieter Swandulla, Kay Ohlendieck
PMID33377090
RÉSUMÉ
This protocol describes the comparative proteomic profiling of the spleen of wild type versus mdx-4cv mouse, a model of dystrophinopathy. We detail sample preparation for bottom-up proteomic mass spectrometry experiments, including homogenization of tissue, protein concentration measurements, protein digestion, and removal of interfering chemicals. We then describe the steps for mass spectrometric analysis and bioinformatic evaluation. For complete details on the use and execution of this protocol, please refer to Dowling et al. (2020).
MATÉRIAUX
Référence du produit
Marque
Description du produit
Sigma-Aldrich
Dodécylsulfate de sodium, BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)