Accéder au contenu
Merck

Bone marrow stromal cell-derived growth inhibitor serves as a stress sensor to induce autophagy.

FEBS letters (2020-01-17)
Jianbin Zhang, Liming Wang, Jian Xu, Yancheng Tang, Bo Huang, Zhifeng Chen, Ting Zhang, Han-Ming Shen, Yihua Wu, Dajing Xia
RÉSUMÉ

Autophagy is an evolutionarily conserved stress response that promotes the lysosomal degradation of intracellular components. The bone marrow stromal cell-derived growth inhibitor (BDGI) functions as a stress sensor which is upregulated by oxidative stress and DNA damage. However, the role of BDGI in autophagic response to certain stresses remains unknown. Here, our results demonstrate that BDGI defines the impact of autophagy induction under stresses. Overexpression of BDGI promotes, while knockdown of BDGI impairs, autophagy. Mechanistically, BDGI localizes to the nucleus and interacts with the transcription factor transcription factor EB to increase the expression of multiple autophagy- and lysosome-related genes. In addition, BDGI regulates autophagy in a p53-dependent manner. Furthermore, BDGI-induced autophagy enables cell survival under stress conditions. Taken together, our study demonstrates that BDGI is a stress sensor that positively regulates autophagy.

MATÉRIAUX
Référence du produit
Marque
Description du produit

Roche
Cocktails d'inhibiteurs de protéases Mini cOmplete, Tablets provided in a glass vial
Sigma-Aldrich
Iodure de propidium, ≥94.0% (HPLC)
Sigma-Aldrich
Anticorps monoclonal anti-β-actine antibody produced in mouse, clone AC-15, ascites fluid
Sigma-Aldrich
Chloroquine diphosphate salt, powder or crystals, 98.5-101.0% (EP)
Sigma-Aldrich
Bafilomycine A1 from Streptomyces griseus, ≥90% (HPLC)
Sigma-Aldrich
Anticorps monoclonal anti-α-tubuline, souris, clone DM1A, purified from hybridoma cell culture
Sigma-Aldrich
Etoposide, synthetic, 95.0-105.0%, powder
Sigma-Aldrich
Anti-LC3B antibody produced in rabbit, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
Milieu d'Eagle modifié par Dulbecco (DMEM) à teneur élevée en glucose, HEPES Modification, With 4500 mg/L glucose, L-glutamine, and 25 mM HEPES, without sodium bicarbonate and pyruvate, powder, suitable for cell culture
Sigma-Aldrich
Anti-SQSTM1 antibody produced in mouse, purified immunoglobulin, buffered aqueous solution