SML3351

Br-DAPI hydrochloride

≥95% (HPLC)

• Synonyme(s) : 2-[4-(Aminoiminomethyl)phenyl]-3-bromo-1H-indole-6-carboximidamide hydrochloride, 3-Bromo-2-(4-carbamimidoylphenyl)-1H-indole-6-carboximidamide
• Formule empirique (notation de Hill): C₁₆H₁₄BrN₅ · xHCl
• Poids moléculaire : 356.22 (free base basis)
• Code UNSPSC : 41116158
• Nomenclature NACRES : NA.77

Propriétés

• Niveau de qualité: 100
• Pureté: ≥95% (HPLC)
• Forme: powder
• Conditions de stockage: desiccated
• Couleur: white to beige
• Solubilité: DMSO: 2 mg/mL, clear
• Température de stockage: 2-8°C
• Chaîne SMILES: BrC1=C(NC2=CC(C(N)=N)=CC=C12)C3=CC=C(C=C3)C(N)=N

Description

Actions biochimiques/physiologiques

Br-DAPI is a DAPI-derived DNA-binding photosensitizer that produces reactive oxygen species (ROS) upon irradiation, resulting in single/double stranded breaks of bound DNA. Light-induced viability reduction is observed among A549 cells treated with Br-DAPI (IC50 = 1.3 μM; 45 min treatment), but not DAPI (IC50 >64 μM) upon photosensitization (365 nm 15 mW/cm2, 4.5 J/cm2 fluence rate). Its high permeation in both Gram-negative and Gram-positive bacteria allows selective light-induced eradication of bacteria over mammalian cells at low concentration (IC50 = 0.29 μM/N99 E. coli and 0.5 μM/B. subtilis) and light intensity (5 min irradiation by 365 nm LED at 15 mW/cm2, 4.5 J/cm2).

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable