S150-05A
Human Skeletal Muscle Cells: HSkMC: Pre-Screened for Insulin & AMPK signaling, adult
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About This Item
Code UNSPSC :
12352204
Produits recommandés
Source biologique
human skeletal muscle (normal limb)
Conditionnement
pkg of 500,000 cells
Mode de croissance
Adherent
Caryotype
2n = 46
Morphologie
Skeletal muscle
Technique(s)
cell culture | mammalian: suitable
Maladie(s) pertinente(s)
muscular dystrophy
Conditions d'expédition
dry ice
Température de stockage
−196°C
Description générale
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Pre-screened Human Skeletal Muscle Cells (HSkMC) are isolated from the skeletal muscle of hamstrings and retain morphological, biochemical, and metabolic characteristics of skeletal muscle. Pre-screened HSkMCs can undergo differentiation to exhibit actin and myosin myofilaments and are specially tested for functional AMPK and Insulin Signaling Pathways.
1. AMPK Signaling Pathway
AMP-activated protein kinase (AMPK) is a major cellular regulator of lipid and glucose metabolism and mediates the metabolic changes associated with exercise. AMPK phosphorylates and inhibits activity of acetyl CoA carboxylase (ACC), the enzyme responsible for making malonyl-CoA which is required for fatty acid chain elongation.
2. Insulin Signaling Pathway
Skeletal muscle is one of major target tissue of insulin action. Upon insulin binding, insulin receptor tyrosine kinases catalyze autophosphorylation of tyrosine residues providing docking sites for downstream signaling components, such as IRS-1 and Grb2, which relay the signaling further into the cell.
Pre-screened Human Skeletal Muscle Cells (HSkMC) are isolated from the skeletal muscle of hamstrings and retain morphological, biochemical, and metabolic characteristics of skeletal muscle. Pre-screened HSkMCs can undergo differentiation to exhibit actin and myosin myofilaments and are specially tested for functional AMPK and Insulin Signaling Pathways.
1. AMPK Signaling Pathway
AMP-activated protein kinase (AMPK) is a major cellular regulator of lipid and glucose metabolism and mediates the metabolic changes associated with exercise. AMPK phosphorylates and inhibits activity of acetyl CoA carboxylase (ACC), the enzyme responsible for making malonyl-CoA which is required for fatty acid chain elongation.
2. Insulin Signaling Pathway
Skeletal muscle is one of major target tissue of insulin action. Upon insulin binding, insulin receptor tyrosine kinases catalyze autophosphorylation of tyrosine residues providing docking sites for downstream signaling components, such as IRS-1 and Grb2, which relay the signaling further into the cell.
Origine de la lignée cellulaire
Muscle
Application
lipid and glucose metabolism, exercise, insulin action, cell signaling
Composants
Basal Medium containing 10% FBS & 10% DMSO
Notes préparatoires
- 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 15 doublings
Procédure de repiquage
Please refer to the HSkMC Culture Protocol.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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