R2631
Pvu II from Proteus vulgaris
Restriction Enzyme
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About This Item
Numéro CAS:
Numéro MDL:
Code UNSPSC :
12352204
Produits recommandés
Qualité
for molecular biology
Forme
buffered aqueous glycerol solution
Concentration
10,000 units/mL
Conditions d'expédition
wet ice
Température de stockage
−20°C
Spécificité
Recognition sequence: 5′-CAG/CTG-3′
Cutting results: a 2-10-fold Pvu II overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Activity not completely destroyed at 65 °C for 15 minutes.
Cutting results: a 2-10-fold Pvu II overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Activity not completely destroyed at 65 °C for 15 minutes.
Application
PvuII is a DNA restriction enzyme used for molecular biology methods to cleave the recognition site 5′-CAG/CTG-3′ to generate DNA fragments with blunt termini.
Autres remarques
Supplied with 10x Restriction Enzyme Buffer SM (B3158).
Comment: This enzyme exhibits star activity when used under conditions of low ionic strength, high enzyme concentration, glycerol concentration >5%, or pH >8.0.
Pvu II does not cut CAGm4CTG or CAGm5CTG.
Pvu II does not cut CAGm4CTG or CAGm5CTG.
Forme physique
Solution in 20 mM Tris-HCl, pH 7.7, 1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.01% Triton X-100 (v/v) at 4 °C
Produit(s) apparenté(s)
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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The proapoptotic gene SIVA is a direct transcriptional target for the tumor suppressors p53 and E2F1.
Fortin, A., et al.
The Journal of Biological Chemistry, 297, 28706-28706 (2004)
K Ozaki-Kuroda et al.
Molecular and cellular biology, 21(3), 827-839 (2001-01-12)
Formin homology (FH) proteins are implicated in cell polarization and cytokinesis through actin organization. There are two FH proteins in the yeast Saccharomyces cerevisiae, Bni1p and Bnr1p. Bni1p physically interacts with Rho family small G proteins (Rho1p and Cdc42p), actin
T R Gingeras et al.
Nucleic acids research, 9(18), 4525-4536 (1981-09-25)
Two novel sequence-specific endonucleases have been isolated from Proteus vulgaris, ATCC 13315. PvuI recognizes the sequence: 5' C G A T decrease C G 3' 3' G C increase T A G C 5' and PvuII recognizes the sequence: 5'
Jaroslav Jelinek et al.
Epigenetics, 7(12), 1368-1378 (2012-10-19)
Genome wide analysis of DNA methylation provides important information in a variety of diseases, including cancer. Here, we describe a simple method, Digital Restriction Enzyme Analysis of Methylation (DREAM), based on next generation sequencing analysis of methylation-specific signatures created by
Saqib H Ansari et al.
Journal of pediatric hematology/oncology, 35(4), e153-e156 (2013-02-08)
β-thalassemia is characterized by impaired β-chain synthesis leading to ineffective erythropoiesis, severe anemia, and a need for blood transfusion. Presence of Xmn I polymorphism (-158 C-T nucleotide change) in γ-globin gene is associated with a higher fetal hemoglobin and a
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