H7523

HEPES

BioXtra, pH 5.0-6.5 (1 M in H₂O), ≥99.5% (titration)

• Synonyme(s) : Acide 4-(2-hydroxyéthyl)pipérazine-1-éthanesulfonique, Acide N-(2-hydroxyéthyl)pipérazine-N′-(2-éthanesulfonique)
• Formule empirique (notation de Hill): C₈H₁₈N₂O₄S
• Numéro CAS: 7365-45-9
• Poids moléculaire : 238.30
• Numéro Beilstein : 883043
• Numéro CE : 230-907-9
• Numéro MDL: MFCD00006158
• Code UNSPSC : 12161700
• ID de substance PubChem : 24895796
• Nomenclature NACRES : NA.25

Propriétés

• Gamme de produits: BioXtra
• Pureté: ≥99.5% (titration)
• Forme: powder
• Impuretés: Insoluble matter, passes filter test
• Résidus de calcination (900 °C): ≤0.1% (as SO₄)
• Perte: ≤1.0% loss on drying, 110°C
• pH: 5.0-6.5 (1 M in H₂O)
• Plage de pH utile: 6.8-8.2
• pKa (25 °C): 7.5
• Solubilité: H₂O: 1 M, clear, colorless
• Traces d'anions: chloride (Cl^-): ≤0.05%
• Traces de cations: Al: ≤0.0005%; As: ≤0.0001%; Ba: ≤0.0005%; Bi: ≤0.0005%; Ca: ≤0.001%; Cd: ≤0.0005%; Co: ≤0.0005%; Cr: ≤0.0005%; Cu: ≤0.0005%; Fe: ≤0.0005%; K: ≤0.005%; Li: ≤0.0005%; Mg: ≤0.0005%; Mn: ≤0.0005%; Mo: ≤0.0005%; Na: ≤0.005%; Ni: ≤0.0005%; Pb: ≤0.0005%; Sr: ≤0.0005%; Zn: ≤0.0005%
• Absorption: ≤0.035 at 280 in H₂O at 1 M; ≤0.045 at 260 in H₂O at 1 M
• Application(s): diagnostic assay manufacturing
• Chaîne SMILES: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• Clé InChI: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Description

Description générale

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

HEPES has been used:

• As a component of homogenisation buffer used in protein sample preparation
• To neutralise PRP (platelet-rich plasma) solution as part of PRP preparation
• As a component in 2X PBS buffer, which is used in phosphate calcium transfection in cell line preparation
• As a supplement in RPMI 1640 medium used to culture human umbilical vein endothelial cells (HUVEC) and in M199 complete medium for ex-vivo treatment of testicular tissue.

Autres remarques

Easily compare specifications for HEPES products with the HEPES specification table.

Informations sur la sécurité

• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)