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GERPN5785

Vistra ECF™ Substrate

Cytiva RPN5785

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About This Item

Code UNSPSC :
41105332
Nomenclature NACRES :
NA.32
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Conditionnement

pkg of 1 kit

Fabricant/nom de marque

Cytiva RPN5785

Couleur

ECF substrate: Off-white/white to yellowish ECF substrate dilution buffer: Colorless

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Description générale

Alkaline phosphatase dephosphorylates ECF™ Substrate to produce a fluorescent product, which localizes at the site of catalysis. Product fluoresces at 540 to 560 nm when excited at the appropriate wavelength.

The following reagents are provided, sufficient for detecting 2500 cm2 membrane: ECF™ Substrate, 60 mL, and dilution buffer.

Application

Vistra ECF™ substrate has been used in Western blotting.[1][2]

Caractéristiques et avantages

  • A fluorescent substrate for alkaline phosphatase-based detection of protein blots, for use with fluorescence scanning equipment such as Storm instruments (see Storm)
  • For use in western blotting, dot, and slot blotting applications
  • Sensitivity comparable to chemiluminescence
  • The wider dynamic range of fluorescence scanning systems compared with film allows visualization and quantitation of weak and dark bands in the same scan
  • Eliminates need for darkroom and film

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

ECF is a trademark of Cytiva

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H318,H315,H373

Code de la classe de stockage

12 - Non Combustible Liquids

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Certificats d'analyse (COA)

Lot/Batch Number

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Consulter la Bibliothèque de documents

Anti-atherosclerotic effects of small molecular weight compounds enhancing eNOS expression and preventing eNOS uncoupling.
Wohlfart P, et al.
Journal of Pharmacology and Experimental Therapeutics (2007)
Cindy Gueguen et al.
PloS one, 9(2), e87367-e87367 (2014-03-04)
Poly(ADP-ribose)polymerase and sirtuin 1 are both NAD(+)-dependent enzymes. In vitro oxidative stress activates poly(ADP-ribose)polymerase, decreases NAD(+) level, sirtuin 1 activity and finally leads to cell death. Poly(ADP-ribose)polymerase hyperactivation contributes to cell death. In addition, poly(ADP-ribose)polymerase inhibition restores NAD(+) level and

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