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A0483

Sigma-Aldrich

Monoclonal Anti-Actin (20-33) antibody produced in rabbit

clone SIG2-AC2, ascites fluid

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

ascites fluid

Type de produit anticorps

primary antibodies

Clone

SIG2-AC2, monoclonal

Poids mol.

antigen ~42 kDa

Espèces réactives

chicken, rat, human

Technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
indirect ELISA: suitable
indirect immunofluorescence: 1:400
western blot: 1:50-1:100 using chicken gizzard total extract

Isotype

IgG

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... ACTB(60)

Description générale

Monoclonal Anti-Actin (20-33) (rabbit IgG) is a rabbit monoclonal derived from the hybridoma SIG2-AC2 produced by the fusion of rabbit myeloma cells and splenocytes from rabbits immunized with a synthetic peptide. Actin is one of the most conserved eukaryotic proteins and is expressed in mammals and birds with at least six isoforms characterized by electrophoresis and amino acid sequence analysis. There are three α-actins (skeletal, cardiac and smooth muscle), one β-actin (β-non-muscle) and two γ-actins (γ-smooth muscle and g-non-muscle).

Immunogène

synthetic peptide corresponding to amino acids 20-33 of actin, attached to a multiple antigen peptide (MAP) backbone.

Application

Monoclonal Anti-Actin (20-33) antibody produced in rabbit has been used in:
  • western blotting
  • enzyme-linked immunosorbent assay (ELISA)
  • immunohistochemistry
  • immunocytochemistry

Forme physique

Supplied as ascites fluid containing 15 mM sodium azide as a preservative.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

PP2A blockade inhibits autophagy and causes intraneuronal accumulation of ubiquitinated proteins
Magnaudeix A, et al.
Neurobiology of Aging, 34(3), 770-790 (2013)
Overexpression of MCPH1 inhibits uncontrolled cell growth by promoting cell apoptosis and arresting the cell cycle in S and G2/M phase in lung cancer cells
Zhou L, et al.
Oncology Letters, 11(1), 365-372 (2016)
The Potential Roles of Actin in The Nucleus
Falahzadeh K, et al.
Cell Journal, 17(1), 7-7 (2015)
Melania Cruciani et al.
Frontiers in immunology, 10, 2622-2622 (2019-11-30)
Understanding Staphylococcus aureus (S. aureus)-host immune system interaction is crucial to meet the tremendous medical need associated with this life-threatening bacterial infection. Given the crucial role of dendritic cells (DC) in dictating immune responses upon microbial challenge, we investigated how
Esther Klaile et al.
Cell cycle (Georgetown, Tex.), 7(20), 3180-3186 (2008-10-10)
In order to maintain genomic integrity during mitosis, cells assemble the mitotic spindle to separate sister chromosomes to the two daughter cells. A variety of motor- and non motor-proteins are involved in the organization and regulation of this complex apparatus.

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