NA0300

GenElute^™ HP Plasmid Maxiprep Kit

sufficient for 10 purifications

• Synonyme(s) : GenElute^™ HP Plasmid Maxiprep Kit
• Code UNSPSC : 41105501
• Nomenclature NACRES : NA.52

Propriétés

• Utilisation: sufficient for 10 purifications
• Niveau de qualité: 200
• Caractéristiques du produit alternatif plus écologique: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• Technique(s): DNA purification: suitable
• Autre catégorie plus écologique: Aligned,
• Température de stockage: 15-25°C

Description

Description générale

The GenElute HP Plasmid Maxiprep Kit offers a simple, rapid, and cost effective method for isolating plasmid DNA from recombinant E. coli cultures. The kit features a filter syringe for the rapid clearing of lysate and a silica binding column designed for either a vacuum or a spin format. Up to 1.2 mg of plasmid DNA can be isolated from a 150 mL overnight culture grown in Luria Broth (LB) medium. Note that the actual yield depends on the strain, the plasmid, and the culture medium used.

An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto a silica membrane in the presence of high salts. Contaminants are removed by two wash steps. Finally, the bound DNA is eluted in Elution Solution (Tris-HCl) or water.

The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, transformation, and transfection.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Application

GenElute^™ HP Plasmid Maxiprep Kit has been used in plasmid DNA extraction of SF91 or MIEV vector[1], apolipoprotein A-V mutant constructs[2], lentiviral vector pLenti6/R4R2/V5-DEST[3],metastasis-associated protein 1 (MTA1)[4] and adipokinetic hormone receptor[5]

Caractéristiques et avantages

• From harvested bacterial culture to pure plasmid DNA in 30 minutes or less
• Up to 1.2 mg of high-copy plasmid DNA
• Flexibility of a vacuum or spin format
• Contains fewer plastic components than other high speed kits, reducing the amount of waste
• No phenol/chloroform extraction or alcohol precipitation required

Autres remarques

For additional information, please see www.sigma-aldrich.com/genelutehp.

Informations légales

GenElute is a trademark of Sigma-Aldrich Co. LLC

Informations sur la sécurité

• Pictogrammes: GHS02,GHS08,GHS05,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H226,H290,H302,H314,H334,H336
• Conseils de prudence: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Classification des risques: Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3
• Organes cibles: Central nervous system
• Code de la classe de stockage: 3 - Flammable liquids
• Point d'éclair (°F): 77.0 °F - closed cup
• Point d'éclair (°C): 25 °C - closed cup