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Merck
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Principaux documents

AB5054

Sigma-Aldrich

Anti-Calretinin Antibody

serum, Chemicon®

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702

Source biologique

rabbit

Niveau de qualité

Forme d'anticorps

serum

Clone

polyclonal

Espèces réactives

human, rat

Fabricant/nom de marque

Chemicon®

Technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Informations sur le gène

human ... CALB2(794)

Description générale

Calretinin is a 29 kDa calcium binding protein that is expressed in central and peripheral nervous system and in many normal and pathological tissues. Calretinin is also considered an important diagnostic aid in the differential diagnosis of cystic and solid ameloblastic tumors. The rat and human calretinin exhibit 98% sequence homology and 91% homology to many other species. Calretinin is generally expressed with two other calcium binding proteins Calbindin D-29 and parvalbumin in CNS and other peripheral tissues. Calretinin antibodies have been used to detect a number of carcinomas by clinicopathological, immunohistochemical techniques including, epithelial malignant mesotheliomas, Ovarian yolk sac tumor, Adenomatoid tumors of the uterus etc.

Spécificité

Reactivity with other species has not been determined
Specific for calretinin. Recognizes both the calcium-bound and calcium-unbound conformations of calretinin by western blots.

Immunogène

Recombinant rat calretinin.

Application

Research Category
Neuroscience
Research Sub Category
Signaling Neuroscience
This Anti-Calretinin Antibody is validated for use in IH, IH(P), WB for the detection of Calretinin.
Western blot:
1:1,000-1:2,000 of a previous lot was used.

Immunohistochemistry:
1:1,000-1:10,000
Antibody works in paraffin embedded tissues.

Optimal working dilutions must be determined by the end user.

Qualité

Immunohistochemistry(paraffin) Analysis:
Calretinin (cat. # AB5054) staining on Human Cerebellum. Tissue pretreated with Citrate, pH 6.0 plus. Antibody was diluted to 1:200 (5.0 µg/mL), using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen as a nuclear and cytoplasmic punctate staining on neurons (brown).
Optimal Staining With Citrate Buffer Epitope Retrieval: Human Cerebellum.

Liaison

Replaces: AB149

Forme physique

Rabbit polyclonal serum contains no preservative.
Unpurified

Stockage et stabilité

Stable for 1 year at -20°C in undiluted aliquots from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Remarque sur l'analyse

Control
Rat brain cortex, Mesothelioma tissue, Human cerebellum.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Repeated phencyclidine in monkeys results in loss of parvalbumin-containing axo-axonic projections in the prefrontal cortex.
Bret A Morrow, John D Elsworth, Robert H Roth
Psychopharmacology (Psychopharmacologia) null
Allison Walter et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 24(9), 3360-3369 (2010-05-06)
This study evaluated the in vivo antitumor activity of grape-derived polyphenols. BALB/c mice were subcutaneously implanted with C26 colon carcinoma cells, and 2 d later they received either solvent or red wine polyphenols (RWPs) (100 mg/kg/d, human equivalent dose approximately
Nitric Oxide Synthase and Calcium-binding Protein-containing Neurons in the Hamster Visual Cortex
Lee, J.-E. et al.
Molecules and Cells, 18, 30-39 (2004)
Calcium-binding Protein Calretinin Immunoreactivity in the Dog Superior Colliculus.
Lee, Jea-Young, et al.
Acta Histochemica Et Cytochemica, 39, 125-138 (2006)
Immunohistochemical and histochemical characteristics of the olfactory system of the guppy, Poecilia reticulata (Teleostei, Poecilidae).
Bettini S, Lazzari M, Ciani F, Franceschini V
Anatomical Record (Hoboken, N.J. : 2007) null

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