14-742-M
FGFR2 (N549H) Protein, active, 10 µg
Active, N-terminal, 6His-tagged, recombinant, human FGFR2 (N549H), amino acids 456-770. For use in Kinase Assays.
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About This Item
Code UNSPSC :
12352202
eCl@ss :
32160405
Nomenclature NACRES :
NA.32
Source biologique
human
Niveau de qualité
Produit recombinant
expressed in baculovirus infected Sf21 cells
Activité spécifique
6250 U/mg
Poids mol.
Mw 38.1 kDa
Fabricant/nom de marque
Upstate®
Technique(s)
activity assay: suitable (kinase)
Numéro d'accès NCBI
Numéro d'accès UniProt
Informations sur le gène
human ... FGFR2(2263)
Description générale
N-terminal, 6His-tagged, recombinant, human FGFR2 (N549H), amino acids 456-770.
Product Source: recombinant, human FGFR2 (N549H), amino acids 456-770, expressed by baculovirus in Sf21 insect cells.
Application
Research Category
Metabolism
Metabolism
Research Sub Category
Cardiovascular Disease
Metabolic Disorders
Cardiovascular Disease
Metabolic Disorders
Actions biochimiques/physiologiques
Protein Target: FGFR2
Target Sub-Family: TK
Qualité
Routinely evaluated by phosphorylation of 500 μM GGEEEEYFELVKKKK.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3μg of active GFGR2 (N549H).
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3μg of active GFGR2 (N549H).
Définition de l'unité
Specific Activity: where one unit of FGFR2 (N549H) activity is defined as 1nmol phosphate incorporated into 500μM GGEEEEYFELVKKKK per minute at 30°C with a final ATP concentration of 100µM.
Forme physique
10 μg of enzyme in 6.3 μL of 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Ni2+/NTA-agarose
Stockage et stabilité
Stable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
Informations légales
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Skin Sens. 1
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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