07-1507

Anti-phospho-Mypt1 (Ser507) Antibody

from rabbit, purified by affinity chromatography

• Synonyme(s) : Protein phosphatase 1 regulatory subunit 12A, Myosin phosphatase-targeting subunit 1, Myosin phosphatase target subunit 1, Protein phosphatase myosin-binding subunit
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: mouse
• Réactivité de l'espèce (prédite par homologie): human (based on 100% sequence homology), chicken (based on 100% sequence homology), bovine (based on 100% sequence homology)
• Technique(s): western blot: suitable
• Numéro d'accès NCBI: NP_002471
• Numéro d'accès UniProt: O14974
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: phosphorylation (pSer507)
• Informations sur le gène: human ... PPP1R12A(4659)

Description

Description générale

Myosin phosphatase targeting subunit 1 (MYPT1) is a regulatory targeting subunit that is thought to be responsible for targeting the holoenzyme to the proper positions in muscle and moving PP1 to myosin. MYPT1 is the major subunit involved in myosin light chain phosphatase (MLCP). It is also essential for vasodilation caused by nitric oxide (NO). A decrease in MYPT1 has been shown to play a role in increased tolerance of NO in pulmonary arteries.

Spécificité

Other homologies: Rat (89% sequence homology).

This antibody recognizes Mypt1 phosphorylated at (Ser507).

Immunogène

Epitope: Phosphorylated (Ser507)

KLH-conjugated linear peptide corresponding to human Mypt1 phosphorylated at (Ser507).

Application

Research Category
Signaling

Cell Structure

Research Sub Category
Developmental Signaling

Muscle Physiology

This Anti-phospho-Mypt1 (Ser507) Antibody is validated for use in WB for the detection of phospho-Mypt1 (Ser507).

Qualité

Evaluated by Western Blot in untreated and PMA-treated NIH/3T3 cell lysates.

Western Blot Analysis: 2 µg/mL of this antibody detected Mypt1 in 10 µg of untreated and PMA-treated NIH/3T3 cell lysates.

Description de la cible

~120 kDa observed.
Uniprot gives a calculated molecular weight of 115 kDa, which can be observed at higher molecular weights due to hyperphosphorylation. Uncharacterized bands appear at ~30 kDa and ~130 kDa in some lysates.

Forme physique

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4) and 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
Untreated and PMA-treated NIH/3T3 cell lysates

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable