The pH optimum for yeast PGI (Product No. P5381) is 7, according to Marchand, M. et al., Eur. J. Biochem., 184, 455-464 (1989).
P5381
Phosphoglucose Isomerase from baker′s yeast (S. cerevisiae)
Type III, ammonium sulfate suspension, ≥400 units/mg protein (biuret)
Synonyme(s) :
D-Glucose-6-phosphate ketol-isomerase, PGI, Phosphosaccharomutase
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112,00 €
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1000 UNITS
112,00 €
5000 UNITS
403,00 €
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2 200,00 €
About This Item
Produits recommandés
Source biologique
bakers yeast
Type
Type III
Forme
ammonium sulfate suspension
Activité spécifique
≥400 units/mg protein (biuret)
Poids mol.
tetramer 119,500 Da±600
Concentration
≥3 mg/mL protein Biuret
Technique(s)
activity assay: suitable
Couleur
white
Numéro d'accès Protein ID
Numéro d'accès UniProt
Température de stockage
2-8°C
Informations sur le gène
yeast ... PGI1(852495)
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Description générale
Application
Phosphoglucose Isomerase from baker′s yeast (S. cerevisiae) has been used:
- to convert fructose-6-phosphate to glucose-6-phosphate during the enzymatic measurement of serum mannose levels.[3]
- as one of the enzymes in the series of four enzyme-coupled reactions to measure phosphomannomutase (PMM) activity.[4]
- to convert fructose to glucose while evaluating fructose content in kudzu roots.[5]
Actions biochimiques/physiologiques
Phosphoglucose Isomerase(PGI) is an enzyme crucial for the interconversion of D-glucose 6-phosphate and D-fructose 6-phosphate. PGI is responsible for the second step of glycolysis and is involved in glucogenesis and pentose phosphate pathway (PPP).[6] It is highly conserved in bacteria and eukaryotes. PGI plays a vital role in the survival of A. fumigatus and demonstrates diverse functions that encompass development, biosynthesis of cell wall glucan, and virulence.[7]Phosphoglucose Isomerase functions as an isomerase, neuroleukin, autocrine motility factor, and a differentiation and maturation mediator.[6]
Définition de l'unité
One unit will convert 1.0 μmole of D-fructose 6-phosphate to D-glucose 6-phosphate per min at pH 7.4 at 25 °C.
Forme physique
Crystalline suspension in 2.6 M (NH4)2SO4 containing 0.1 mM magnesium acetate, pH 7.0
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves
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Lot/Batch Number
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Les clients ont également consulté
Regulation of phosphoglucose isomerase/autocrine motility factor activities by the poly(ADP-ribose) polymerase family-14
Yanagawa T, et al.
Cancer Research, 67(18), 8682-8689 (2007)
S L Lowe et al.
The Journal of biological chemistry, 250(1), 94-99 (1975-01-10)
Bakers' yeast phosphoglucose isomerase was studied by both chemical and physical methods to determine its submit structure. Gel filtration in 6 M guanidine HCl as well as acrylamide gel electrophoresis of sodium dodecyl sulfatedentured phosphoglucose isomerase showed two speices corresponding
Phosphoglucose Isomerase Is Important for Aspergillus fumigatus Cell Wall Biogenesis
Zhou Y, et al.
mBio, 13(4) (2022)
Kudzu [Pueraria montana (Lour.) Merr. Variety lobata: A new source of carbohydrate for bioethanol production
Sage RF, et al.
Biomass and Bioenergy, 33, 57-61 (2009)
Yeast Models of Phosphomannomutase 2 Deficiency, a Congenital Disorder of Glycosylation
Lao JP, et al.
G3 (Bethesda, Md.), 9(2), 413-423 (2019)
Articles
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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What is the pH optimum of Phosphoglucose Isomerase from baker's yeast (e.g., Product No. P5381)?
1 réponse-
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What is the substrate for Phosphoglucose Isomerase from baker's yeast (e.g., Product No. P5381)?
1 réponse-
The substrate for PGI is D-fructose-6-phosphate, according to our Enzyme Assay procedure.
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What is the KM value for yeast PGI (Product No. P5381) with D-Fructose-6-phosphate as substrate, and what does that mean?
1 réponse-
KM is the concentration of substrate that leads to half-maximal velocity. The KM value for yeast PGI (Product No. P5381) with D-Fructose-6-phosphate as substrate is 0.167, according to Marchand, M. et al., Eur. J. Biochem., 184, 455-464 (1989).
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What is the typical protein concentration for Product No. P5381 (PGI)?
1 réponse-
The protein concentration of Product No. P5381 (PGI) can vary from lot-to-lot. It is typically in the range of 5-20 mg protein per mL. The lot-specific concentration will be given on the certificate of analysis.
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What is the Department of Transportation shipping information for this product?
1 réponse-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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