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GALS

Sigma-Aldrich

β-Galactosidase Reporter Gene Staining Kit

Synonyme(s) :

β-galactosidase, LacZ

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1 KIT
545,00 €

545,00 €

Date d'expédition estimée le29 mai 2025

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1 KIT
545,00 €

About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.32

545,00 €

Date d'expédition estimée le29 mai 2025

Utilisation

 kit sufficient for 100 tests (using a 3.5 cm dish)

Niveau de qualité

200

Technique(s)

microbe id | staining: suitable

Conditions d'expédition

dry ice

Température de stockage

−20°C

Description générale

β-galactosidase (β-gal) catalyzes the conversion of X-gal to galactose and 5-bromo-4-chloro-3-hydroxyindole.[1] The functionality of β-gal is indirectly measured by the blue precipitate of dimeric 5-bromo-4-chloro-3-hydroxyindole in the presence of potassium ferricyanide or ferrocyanide.[1] The colorimetric detection methods also use other substrates of β-gal like O-nitrophenyl-β-D-galactopyranoside (ONPG). β-gal is a cost-effective, simple and standard reporter system.[2] The Galactosidase Reproter Gene Staining Kit provides a method for determining the percentage of cells transfected with the plasmid expressing the β-galactosidase gene, LacZ. It can also be used to visualize the specific expression of the reporter gene in tissue sections by microscopy.

Application

β-Galactosidase Reporter Gene Staining Kit has been used for histochemical staining of animal cells or tissues such as:
  • sagittal brain sections in histoenzymology[3]
  • pancreas or colon sections in β-galactosidase reporter gene assay[4]
  • mice E10.5 -11.5 embryo and embryo sections[5]

Autres remarques

The substrate used in this kit is X-Gal (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside) which results in an indigo blue-colored staining of transfected cells and tissues. This test provides a method for determining the percentage of transfected cells that are expressing LacZ or for microscopically visualizing reporter gene expression in tissue sections.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

94433

β-Galactosidase stain

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

Organes cibles

Respiratory system

Risques supp

EUH032

Code de la classe de stockage

3 - Flammable liquids

Point d'éclair (°F)

132.8 °F

Point d'éclair (°C)

56 °C

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Certificats d'analyse (COA)

Lot/Batch Number
0000327711
0000177219
029M4161V
11180807
117M4124V

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Wendy Phillips et al.
Methods in molecular biology (Clifton, N.J.), 549, 137-155 (2009-04-21)
Animal models of neurodegenerative disease are excellent tools for studying pathogenesis and therapies including cellular transplantation. In this chapter, we describe different models of Huntington's disease and Parkinson's disease, stereotactic surgery (used in creation of lesion models and transplantation) and
beta-Galactosidase assay
Smale ST
Cold Spring Harbor Protocols, 2010, pdb-prot5423 (2010)
Snir Yehuda et al.
Biogerontology, 18(4), 525-533 (2017-03-03)
The role of telomere shortening in the induction of replicative cellular senescence (CS) is well known and as a result, the involvement of telomerase and in particular its catalytic subunit, the telomerase reverse transcriptase (TERT) in CS has also been
Virginia M Stone et al.
Diabetologia, 57(6), 1182-1191 (2014-03-26)
The NEFA-responsive G-protein coupled receptor 120 (GPR120) has been implicated in the regulation of inflammation, in the control of incretin secretion and as a predisposing factor influencing the development of type 2 diabetes by regulation of islet cell apoptosis. However
Hyo Young Kim et al.
Scientific reports, 5, 11719-11719 (2015-06-27)
Cell penetrating peptides (CPPs) are peptides that can be translocated into cells and used as a carrier platform for the intracellular uptake of cargo molecules. Subject to the source of CPP sequences and their positively charged nature, the cytotoxicity and
Afficher tous les articles scientifiques apparentés

Questions

  1. Is the GALS kit compatible with fixed, frozen mouse brain that used a totally different fixative for 4 days or longer?

    1 réponse

    1. The GALS kit, identified by product number F1797, is not tested or recommended for use with a mouse brain that has been fixed using a different fixative for 4 days or longer. The standard kit procedure fixes cells for only 10 minutes, whereas the fixation period used for the mouse brain is 396 times longer than what is recommended in the kit protocol. The kit was never tested for use on a mouse brain fixed using another fixative. Therefore, it is uncertain whether the fixation used for the mouse brain might produce acceptable results

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