D1946

Diaminofluorescein-FM diacetate

≥98% (HPLC)

• Synonyme(s) : 4-Amino-5-(N-methylamin)-3′,6′-bis(acetyloxy), DAF-FM DA
• Formule empirique (notation de Hill): C₂₅H₁₈F₂N₂O₇
• Numéro CAS: 254109-22-3
• Poids moléculaire : 496.42
• Numéro MDL: MFCD16872050
• Code UNSPSC : 12352200
• ID de substance PubChem : 329798717
• Nomenclature NACRES : NA.77

Propriétés

• Niveau de qualité: 100
• Essai: ≥98% (HPLC)
• Forme: film
• Conditions de stockage: desiccated
• Couleur: colorless to yellow
• Fluorescence: λ_ex 500 nm; λ_em 515 nm in DMSO
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Chaîne SMILES: CNc1ccc2c(c1N)C(=O)OC23c4cc(F)c(OC(C)=O)cc4Oc5cc(OC(C)=O)c(F)cc35
• InChI: 1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3
• Clé InChI: BEVHTVRRVVEMEF-UHFFFAOYSA-N

Description

Application

DAF-FM DA has been used to measure the nitric oxide (NO) levels in liver tissue homogenates. It has also been used in NO staining for detection of NO from human umbilical vein endothelial cells (HUVECs).

Actions biochimiques/physiologiques

DAF-FM DA is a cell-permeable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM and DAF-FM DA are more sensitive to nitric oxide (NO) compared with DAF-2 and more photo-stable and less pH sensitive. DAF-FM DA permeates well into living cells and is rapidly transformed into water-soluble DAF-FM by cytosolic esterases.

Caractéristiques et avantages

This compound is featured on the Nitric Oxide Synthases page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

Attention

Hygroscopic

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable