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A2765

Sigma-Aldrich

Azocasein

protease substrate, chromogenic, powder

Synonyme(s) :

Sulfanilamide-azocasein

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About This Item

Numéro CAS:
102110-74-7
Numéro MDL:
MFCD00130592
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.32

product name

Azocasein, protease substrate

Source biologique

bovine

Forme

powder

Technique(s)

ligand binding assay: suitable

Solubilité

water: 5 mg/mL, clear, orange to very deep orange

ε (coefficient d'extinction)

≥25 at 440 nm in 0.1 M NaOH at 1%

Température de stockage

2-8°C

Description générale

Azocasein is a chromogenic derivative of casein. Protease degrades azocasein to yield TCA-soluble azopeptides with high UV-absorbance. This azocasein assay is widely employed to estimate the protease production by bacterial fermentation on synthetic substrates from glucose and inorganic salts.

Application

Azocasein has been used as a substrate for determination of protease activity.
Azocasein is a nonspecific protease substrate. Hydrolysis of the casein releases the azo dye into the media where it is detected by absorbance at 440 nm.
Azocasein is an inflammatory agent that is used to induce amyloid A amylooidosis in experimental animals.

Liaison

View more information on azocasein protease assay at www.sigma-aldrich.com/enzymeexplorer

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Isabel Morales-Belpaire et al.
Water research, 42(17), 4449-4456 (2008-09-03)
Laboratory data on the behaviour of the pathogenic form of the prion protein (PrP(Sc)) in environmental matrices such as sewage sludge is scarce. Direct experiments with this misfolded protein require strict safety measures, pathogen class-3 facilities and costly reagents. However
Yosuke Tashiro et al.
Journal of bacteriology, 191(24), 7509-7519 (2009-10-20)
The opportunistic human bacterial pathogen Pseudomonas aeruginosa produces membrane vesicles (MVs) in its surrounding environment. Several features of the P. aeruginosa MV production mechanism are still unknown. We previously observed that depletion of Opr86, which has a role in outer
Isolation and characterization of chitinolytic Streptomyces sp. MT7 and its antagonism towards wood-rotting fungi
Nagpure, A, et al.
Annals of Microbiology, 64(2), 531-541 (2014)
Recombinant proteins from plants: production and isolation of clinically useful compounds, 3 (1998)
Nicolas Oswaldo Gomez et al.
Molecular microbiology, 115(1), 84-98 (2020-09-09)
To overcome the metal restriction imposed by the host's nutritional immunity, pathogenic bacteria use high metal affinity molecules called metallophores. Metallophore-mediated metal uptake pathways necessitate complex cycles of synthesis, secretion, and recovery of the metallophore across the bacterial envelope. We
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