What Does it Cover?
Cell lines remain the workhorses of laboratory research, both in academic and industrial settings. This webinar will focus on best practice in selecting and maintaining cell lines for research, to ensure research outputs are relevant and reproducible. Features of cell lines that might be used in selection will be discussed. This will include monolayer or suspension culture; passage number; doubling time; immortalisation; karyotype; receptors; expression profiling and use of bioinformatics tools like the cancer cell line encyclopaedia.
Using breast cancer as an example, we will discuss more specifically how to choose the most relevant cell line model to address a particular research question. Finally our speaker will describe how to use (and access) human material to help develop more clinically relevant cell culture systems to model human disease and the role that biobanks play in this process.
What Will You Learn?
- Which features are important in choosing a cell line for scientific research (featured example: breast cancer research)
- How to improve the reliability, reproducibility and relevance of your research
Speaker
Valerie Speirs
University of Leeds
Professor of Experimental Pathology and Oncology
Professor Valerie Speirs holds a BSc (Hons) Zoology degree from the University of Aberdeen and carried out her PhD at the University of Glasgow under the tutelage of Ian Freshney, widely known as a doyenne in cell culture. This helped spark her interest in developing improved cell culture systems to model human disease, which she has maintained throughout a career which has taken her to Toronto, Liverpool, Hull and Leeds where she is currently Professor of Experimental Pathology & Oncology.
Cell culture and analysis
- Microbial cell culture
Durée:56min
Langue:English
Session 1:présenté(e) June 25, 2015
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