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  • Triacylglycerols and aliphatic alcohols from fruits of three Tunisian Pistacia lentiscus populations.

Triacylglycerols and aliphatic alcohols from fruits of three Tunisian Pistacia lentiscus populations.

Journal of the science of food and agriculture (2014-09-13)
Hajer Trabelsi, Justin Renaud, Wahid Herchi, Sadok Boukhchina, Paul Mayer
ABSTRACT

The search for other sources of vegetable oils by the exploitation and the enhancement of other oil plants will be needed to meet the demands of the international market. This study aims to determine the triacylglycerol (TAG) molecular species and aliphatic alcohol compositions of unexploited fruits of three Tunisian Pistacia lentiscus (lentisc) populations from the Korbous, Tebaba and Rimel areas of Tunisia. Results show that the content of total TAG varies from 738.32 mg g(-1) of total lipid in the Tebaba population to 981.15 mg g(-1) of total lipid in the Korbous population. Furthermore, 14 species of TAG were detected in the three studied populations. In addition, 13 aliphatic compounds were identified and classified into two groups: (1) aliphatic alcohols with fewer than 20 carbon atoms (hexadecanol, heptadecanol, (Z)-octadec-9-en-1-ol, octadecanol and nonadécanol); and (2) the policosanol group (eicosenol, docosenol, docosanol tetracosanol, hexacosanol octacosanol and triacontanol). The Tebaba population showed a distinct composition compared to Korbous and Rimel where heptadecanol is the major compound. Quantitatively, the most abundant TAG species are those constituted by palmitic, oleic and/or linoleic acid. Furthermore, the significant difference observed at the oil composition is associated with a remarkable station effect.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Acetic acid-12C2, 99.9 atom % 12C
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Chlorotrimethylsilane, produced by Wacker Chemie AG, Burghausen, Germany, ≥99.0% (GC)
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Sodium sulfate, BioUltra, anhydrous, ≥99.0% (T)
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Sodium sulfate, ≥99.99% trace metals basis
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Chloroform, anhydrous, ≥99%, contains 0.5-1.0% ethanol as stabilizer
Sigma-Aldrich
Chloroform, anhydrous, contains amylenes as stabilizer, ≥99%
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Acetic acid, natural, ≥99.5%, FG
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Acetic acid, ≥99.5%, FCC, FG
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Triethylamine, for protein sequence analysis, ampule, ≥99.5% (GC)
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Triethylamine, for amino acid analysis, ≥99.5% (GC)
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Triethylamine, BioUltra, ≥99.5% (GC)
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Acetic acid, for luminescence, BioUltra, ≥99.5% (GC)
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Diethyl ether
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Chlorotrimethylsilane, purified by redistillation, ≥99%
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Triethylamine, ≥99.5%
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Diethyl ether, contains 1 ppm BHT as inhibitor, anhydrous, ≥99.7%
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Isopropyl alcohol, ≥99.7%, FCC, FG
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Triethylamine, puriss. p.a., ≥99.5% (GC)
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Triethylamine, ≥99%
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Sodium sulfate, BioXtra, ≥99.0%
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Sodium sulfate, ≥99.0%, suitable for plant cell culture
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Chloroform, ≥99%, PCR Reagent, contains amylenes as stabilizer
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Triethylamine, ≥99.5%
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Chlorotrimethylsilane, ≥98.0% (GC)
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2-Propanol, electronic grade, 99.999% trace metals basis
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5α-Androstan-17β-ol-3-one, purum, ≥99.0% (TLC)
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2-Propanol, BioUltra, for molecular biology, ≥99.5% (GC)
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2-Propanol, anhydrous, 99.5%
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1-Eicosanol, 98%
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Chlorotrimethylsilane solution, 1.0 M in THF