Skip to Content
Merck
  • Protective Role of Ternatin Anthocyanins and Quercetin Glycosides from Butterfly Pea (Clitoria ternatea Leguminosae) Blue Flower Petals against Lipopolysaccharide (LPS)-Induced Inflammation in Macrophage Cells.

Protective Role of Ternatin Anthocyanins and Quercetin Glycosides from Butterfly Pea (Clitoria ternatea Leguminosae) Blue Flower Petals against Lipopolysaccharide (LPS)-Induced Inflammation in Macrophage Cells.

Journal of agricultural and food chemistry (2015-06-30)
Vimal Nair, Woo Young Bang, Elisa Schreckinger, Nuri Andarwulan, Luis Cisneros-Zevallos
PMID26120869
ABSTRACT

Twelve phenolic metabolites (nine ternatin anthocyanins and three glycosylated quercetins) were identified from the blue flowers of Clitoria ternatea by high-performance liquid chromatography diode array detection and electrospray ionization/mass spectrometry (HPLC-DAD-ESI/MS(n)). Three anthocyanins not reported in this species before show fragmentation pattern of the ternatin class. Extracts were fractionated in fractions containing flavonols (F3) and ternatin anthocyanins (F4). In general, C. ternatea polyphenols showed anti-inflammatory properties in lipopolysaccharide (LPS)-induced inflammation in RAW 264.7 macrophage cells with distinct molecular targets. Flavonols (F3) showed strong inhibition of COX-2 activity and partial ROS suppression. On the other hand, the ternatin anthocyanins (F4) inhibited nuclear NF-κB translocation, iNOS protein expression, and NO production through a non-ROS suppression mechanism. Accordingly, quercetin glycosides and ternatin anthocyanins from the blue flower petals of C. ternatea may be useful in developing drugs or nutraceuticals for protection against chronic inflammatory diseases by suppressing the excessive production of pro-inflammatory mediators from macrophage cells.

MATERIALS
Product Number
Brand
Product Description
717975
Sigma-Aldrich
Methanol, NMR reference standard
Pricing and availability is not currently available.
608203
Sigma-Aldrich
D-Glucose-12C6, 16O6, 99.9 atom % 16O, 99.9 atom % 12C
Pricing and availability is not currently available.
322415
Sigma-Aldrich
Methanol, anhydrous, 99.8%
Pricing and availability is not currently available.
R9504
Sigma-Aldrich
(−)-Riboflavin, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥98%
Pricing and availability is not currently available.
D6883
Sigma-Aldrich
2′,7′-Dichlorodihydrofluorescein diacetate, ≥97%
Pricing and availability is not currently available.
S5761
Sigma-Aldrich
Sodium bicarbonate, powder, BioReagent, for molecular biology, suitable for cell culture, suitable for insect cell culture
Pricing and availability is not currently available.
N6639
Sigma-Aldrich
Nitrotetrazolium Blue chloride, powder, electrophoresis grade
Pricing and availability is not currently available.
N6876
Sigma-Aldrich
Nitrotetrazolium Blue chloride, ≥90.0% (HPLC)
Pricing and availability is not currently available.
G6152
Sigma-Aldrich
D-(+)-Glucose, suitable for mouse embryo cell culture, ≥99.5% (GC)
Pricing and availability is not currently available.
G5767
Sigma-Aldrich
D-(+)-Glucose, ACS reagent
Pricing and availability is not currently available.
G7528
Sigma-Aldrich
D-(+)-Glucose, ≥99.5% (GC), BioXtra
Pricing and availability is not currently available.
G8270
Sigma-Aldrich
D-(+)-Glucose, ≥99.5% (GC)
Pricing and availability is not currently available.
R4500
Sigma-Aldrich
(−)-Riboflavin, from Eremothecium ashbyii, ≥98%
Pricing and availability is not currently available.
C3878
Sigma-Aldrich
Chlorogenic acid, ≥95% (titration)
Pricing and availability is not currently available.
G5146
Sigma-Aldrich
D-(+)-Glucose, Hybri-Max™, powder, BioReagent, suitable for hybridoma
Pricing and availability is not currently available.
S6297
Sigma-Aldrich
Sodium bicarbonate, BioXtra, 99.5-100.5%
Pricing and availability is not currently available.
49139
Sigma-Aldrich
D-(+)-Glucose, BioUltra, anhydrous, ≥99.5% (sum of enantiomers, HPLC)
Pricing and availability is not currently available.
G7021
Sigma-Aldrich
D-(+)-Glucose, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.5%
Pricing and availability is not currently available.
R7649
Sigma-Aldrich
(−)-Riboflavin, meets USP testing specifications
Pricing and availability is not currently available.
721956
Sigma-Aldrich
Methanol solution, NMR reference standard, 4% in methanol-d4 (99.8 atom % D), NMR tube size 3 mm × 8 in.
Pricing and availability is not currently available.
606723
Sigma-Aldrich
Methanol-12C, 99.95 atom % 12C
Pricing and availability is not currently available.
733466
Sigma-Aldrich
Acetonitrile, electronic grade, 99.999% trace metals basis
Pricing and availability is not currently available.
D2438
Sigma-Aldrich
Dimethyl sulfoxide, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
Pricing and availability is not currently available.
41639
Sigma-Aldrich
Dimethyl sulfoxide, BioUltra, for molecular biology, ≥99.5% (GC)
Pricing and availability is not currently available.
D1435
Sigma-Aldrich
Dimethyl sulfoxide, meets EP testing specifications, meets USP testing specifications
Pricing and availability is not currently available.
P4557
Sigma-Aldrich
Phenol solution, Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, BioReagent, for molecular biology
Pricing and availability is not currently available.
D4540
Sigma-Aldrich
Dimethyl sulfoxide, ≥99.5% (GC), suitable for plant cell culture
Pricing and availability is not currently available.
D2650
Sigma-Aldrich
Dimethyl sulfoxide, Hybri-Max™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
Pricing and availability is not currently available.
S8501
Sigma-Aldrich
Sucrose, ≥99.5% (GC)
Pricing and availability is not currently available.
S5016
Sigma-Aldrich
Sucrose, ACS reagent
Pricing and availability is not currently available.