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Merck

Monitoring therapeutic monoclonal antibodies in brain tumor.

mAbs (2014-12-09)
Rima Ait-Belkacem, Caroline Berenguer, Claude Villard, L'Houcine Ouafik, Dominique Figarella-Branger, Alain Beck, Olivier Chinot, Daniel Lafitte
ABSTRACT

Bevacizumab induces normalization of abnormal blood vessels, making them less leaky. By binding to vascular endothelial growth factor, it indirectly attacks the vascular tumor mass. The optimal delivery of targeted therapies including monoclonal antibodies or anti-angiogenesis drugs to the target tissue highly depends on the blood-brain barrier permeability. It is therefore critical to investigate how drugs effectively reach the tumor. In situ investigation of drug distribution could provide a better understanding of pharmacological agent action and optimize chemotherapies for solid tumors. We developed an imaging method coupled to protein identification using matrix-assisted laser desorption/ionization mass spectrometry. This approach monitored bevacizumab distribution within the brain structures, and especially within the tumor, without any labeling.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Acetonitrile, ReagentPlus®, 99%
Sigma-Aldrich
Ethanol, purum, absolute ethanol, denaturated with 2% 2-butanone, A15 MEK1, ≥99.8% (based on denaturant-free substance)
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Ethanol, purum, fine spirit, denaturated with 2% 2-butanone, F25 MEK1, ~96% (based on denaturant-free substance)
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Trifluoroacetic acid, ReagentPlus®, 99%
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Acetonitrile, anhydrous, 99.8%
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Ethanol, tested according to Ph. Eur.
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Acetonitrile, analytical standard
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Ethanol, for residue analysis
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Ethyl alcohol, Pure, 190 proof, ACS spectrophotometric grade, 95.0%
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Ethanol, BioUltra, for molecular biology, ≥99.8%, (absolute alcohol, without additive, A15 o1)
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Acetonitrile, electronic grade, 99.999% trace metals basis
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Ethanol, purum, absolute ethanol, denaturated with 4.8% isopropanol, A15 IPA1, ≥99.8% (based on denaturant-free substance)
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Ethanol, purum, fine spirit, denaturated with 4.8% methanol, F25 METHYL1, ~96% (based on denaturant-free substance)
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Trifluoroacetic acid, puriss. p.a., suitable for HPLC, ≥99.0% (GC)
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Ultrapure Acetonitrile
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Acetonitrile, suitable for HPLC, gradient grade, ≥99.9%
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Trifluoroacetic acid, suitable for HPLC, ≥99.0%
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Acetonitrile, HPLC Plus, ≥99.9%
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Acetonitrile, suitable for HPLC, gradient grade, ≥99.9%
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Residual Solvent Class 2 - Acetonitrile, United States Pharmacopeia (USP) Reference Standard
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Dehydrated Alcohol, Pharmaceutical Secondary Standard; Certified Reference Material