- Chitin Extraction and Content Measurement in Magnaporthe oryzae.
Chitin Extraction and Content Measurement in Magnaporthe oryzae.
Bio-protocol (2017-03-05)
Xinyu Liu, Zhengguang Zhang
PMID34458477
ABSTRACT
Chitin is a linear polysaccharide composed of β (1→4)-linked N-acetylglucosamine (GlcNAc) residues. In fungi, chitin is an important component of the cell wall. Here, we provide a protocol to measure the chitin content of fungal cells using Magnaporthe oryzae as an example.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Chitinase from Streptomyces griseus, lyophilized powder (essentially salt free), ≥200 units/g solid